贵州地区蜘蛛抱蛋属植物ITS1区序列分析及其亲缘关系研究  

Molecular Analysis of Aspidistra Ker-Gawl in Guizhou on ITS Sequence

作　　者：黄海[1] 王安仙[1] 刘杨[1] 张筠源[1] 何顺志[2] 

机构地区：[1]贵阳医学院医学检验系,贵阳550004 [2]贵阳中医学院药学系,贵阳550002

出　　处：《贵州科学》2010年第2期50-52,共3页Guizhou Science

基　　金：国家自然科学基金项目(30660224);[筑科农(2009)3-048]

摘　　要：从贵州不同地区采集的5株蜘蛛抱蛋属植物,采用直接测序对其核糖体DNA内间隔区(ITS)序列进行测定,用于分析蜘蛛抱蛋属植物间的亲缘关系。测序结果提示该植物的ITS1区域的GC%在62.7%～68.85%之间,有121个变化位点,提示可用于对形状相似的蜘蛛抱蛋属植物进行分子生物学分类和用于系统发育分析。The ITS sequence in 5 strains of Aspidistra Ker-Gawl collected from Guizhou were determined for the phylogenetic analysis.All of sequences shown that there are 121 variant sites in the GC%contents ranging from 62.7%to 68.85%.The results suggested that ITS sequence comparison was suitable for the molecular classification and phylogenetic analysis.

关 键 词：蜘蛛抱蛋属 ITS序列 百合科 

分 类 号：Q943[生物学—植物学]