10种中药提取物对耐药金黄色葡萄球菌抑菌作用的研究  

Inhibition Effects of 10 kinds of Traditional Chinese Medicine Extracts on Drug-resistant S.aureus

作　　者：李伟奇[1,2] 刘湘新[3] 李俊超[3] 贺建华[1,2] 

机构地区：[1]湖南农业大学食品科技学院,湖南长沙410128 [2]湖南农业大学动物科技学院,湖南长沙410128 [3]湖南农业大学动物医学院,湖南长沙410128

出　　处：《动物医学进展》2010年第9期41-46,共6页Progress In Veterinary Medicine

基　　金：国际科技合作计划项目(2009DFA32350);国家科技支撑计划项目(2007BAQ01047)

摘　　要：为了解研究中药提取物对耐药菌的抑菌作用,采用纸片法和倍比稀释法研究10种中药水提取液对耐氟喹诺酮类药物金黄色葡萄球菌的体外抑菌作用;用点种法和药敏纸片法研究耐药抑转作用。结果显示,10种中药水提物中黄芩水提取物对耐氟喹诺酮类药物金黄色葡萄球菌抑制效果最明显。耐药性抑制试验结果表明,只有黄芩水提取物对Sa1的耐药性有明显的抑制作用,抑制率达65%,但对其他菌株的抑制作用则表现不明显;药敏纸片法显示黄芩对细菌耐药性有最强抑制作用。表明中药水提取物对耐氟喹诺酮类药物金黄色葡萄球菌有抑制作用,且部分药物抑菌作用明显;黄芩水提取物对Sa1菌株具有耐药逆转作用。The disquisition studyies on the inhibition effect of herb extracts on S.aureus resistant to the fluoroquinolones were conducted.The inhibition effects of water soluble extract of 10 kinds of Chinese medicine on S.aureus resistant to the fluoroquinolones were investigated by paper disk method and tube dilution method in vitro,and the MIC of scutellarias extracts on pathogenic bacteria was measured.The inhibition rate of 10 kinds Chinese medicine extracts to fluoroquinolone-resistant S.aureus was measured by the point to point grow method and drug sensitive test.The results showed the water soluble extracts of scutellarias had biggst inhibition effect in10 kinds of Chinese medicine,it showed significant inhibition effect to fluoroquinolone-resistant of Sa1,the inhibition rate is 65%.In the drug sensitive test,the water soluble extracts of scutellarias shows a great inhibition effect too.It indicated that water soluble extracts of 10 kinds of Chinese medicine had significantly bacteria-inhibition effect to drug-resistant S.aureus,some medicines showed evident inhibition effect.Scutellarias extract had resistant-reverse effect to Sa1 resistant ot fluoroquinolones.

关 键 词：水提取物 耐药金色葡萄球菌 体外抑菌试验 中药 

分 类 号：S853.7[农业科学—临床兽医学]