NREL法测定木质纤维素原料组分的含量  

Investigation on the determination of lignocellulosics components by NREL method

作　　者：张红漫[1] 郑荣平[1] 陈敬文[2] 黄和[2] 

机构地区：[1]南京工业大学理学院,南京210009 [2]南京工业大学制药与生命科学学院,南京210009

出　　处：《分析试验室》2010年第11期15-18,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20876078);国家863计划(2009AA02Z08);中石化项目(207035)资助

摘　　要：对美国国家可再生能源实验室(NREL)方法定量木质纤维素原料中纤维素、半纤维素及木质素的实验条件进行了研究。72%浓硫酸水解1h、4%稀硫酸水解45min可将玉米秸秆中的纤维素、半纤维素降解为可用HPLC定量的单糖,适宜的样品添加量为0.5g。同时,将NREL法与国标法进行了比对,两种方法的测定结果基本一致。NREL法测定玉米秸秆中纤维素、半纤维素及木质素的含量分别为33.90%、23.89%和15.84%。The experimental conditions of National Renewable Energy Lab (NREL) method for quantifying cellulose,hemicellulose and lignin of lignocellulosics had been investigated in detail.It was showed that the cellulose and semicellulose of corn stover could be degraded to mono-sugars quantified by HPLC under the condition of 72% concentrated sulphuric acid treating 1 h and 4% dilute acid treating 45 min with the appropriate amount of 0.5 g of sample.Meanwhile,the determination results of cellulose,hemicellulose and lignin obtained by NERL method were simultaneously compared those that by national standard method,and the results exhibited no remarkable differences.The contents of cellulose,hemicellulose and lignin of corn determined by NREL method were 33.90%,23.89 % and 15.84% respectively.

关 键 词：NREL法 纤维素 半纤维素 玉米秸秆 定量分析 

分 类 号：TQ352.4[化学工程]