脑脊液14-3-3蛋白检测对隐球菌性脑膜炎的意义  

Significance of test of 14-3-3 protein in the cerebrospinal fluid in cryptococcal meningitis

作　　者：朱艳霞[1] 何俊瑛[1] 卜晖[1] 洪坤[1] 刘士甫[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000

出　　处：《脑与神经疾病杂志》2010年第5期358-361,共4页Journal of Brain and Nervous Diseases

摘　　要：目的评价脑脊液酪氨酸3-加单氧酶/色氨酸5-加单氧酶激活蛋白(14-3-3蛋白)含量的变化与隐球菌性脑膜炎(CM)患者病情及预后之间的联系。方法从16例隐球菌性脑膜炎患者和18例对照组中获取脑脊液标本,进行脑脊液细胞学检测,并用ELISA法检测脑脊液中14-3-3蛋白含量。结果隐球菌性脑膜炎患者脑脊液中14-3-3蛋白含量较对照组增高,P<0.05。在隐球菌性脑膜炎患者中,有7例患者经过抗真菌治疗症状好转,脑脊液中的隐球菌被清除,但14-3-3蛋白并没有随着治疗的好转及隐球菌的清除有显著下降。结论隐球菌性脑膜炎患者脑脊液中14-3-3蛋白含量较对照组明显增高,且不会在短期内随着治疗的进行和临床症状的改善而显著下降。Objective Evaluation of cerebrospinal fluid 14-3-3 proteins content changes in patients with cryptococcal meningitis and the link between prognosis.Methods We obtained cerebrospinal fluid specimens from 16 cases of cryptococcal meningitis patients and 18 cases of patients with control cases,first,we detected cerebrospinal fluid cytology,then detected 14-3-3 protein content in cerebrospinal fluid by ELISA.Results The 14-3-3 protein content in cerebrospinal fluid in patients with cryptococcal meningitis is much higher than the control group,and there is statistical significance.In patients with cryptococcal meningitis,there are 7 patients whose clinical symptoms improved after anti-fungal treatment,and cryptococcal in cerebrospinal fluid was cleared,but the 14-3-3 protein have not decrease significantly.Conclusion 14-3-3 protein levels in cerebrospinal fluid in patients with cryptococcal meningitis are higher than the control group and did not decline in the near future with the improvement of treatment and conditions.

关 键 词：隐球菌性脑膜炎 脑脊液 脑脊液细胞学 14-3-3蛋白 

分 类 号：R519[医药卫生—内科学]