空肠弯曲菌cdtA及其高抗原肽段的原核重组质粒的构建和表达  

Construction and expression of cytolethal distending toxin cdtA and its peptids of highly antigenicity protein of campylobacter jejuni in Ecoli

作　　者：刘卫卫[1] 白欣立[2] 李震中[1] 张军峰[3] 李鑫[1] 赵子春[1] 李春岩[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000 [2]河北医科大学第二医院儿科,石家庄050000 [3]中国人民解放军二六零医院神经内科

出　　处：《脑与神经疾病杂志》2010年第5期333-336,共4页Journal of Brain and Nervous Diseases

基　　金：国家自然科学基金(81072481);国家高技术研究发展计划(863计划2006AA02A208)

摘　　要：目的空肠弯曲菌细胞扩张毒素cdtA及其高抗原肽段蛋白人工表达。方法使用PCR方法获得cdtA、cdtA1、cdtA2编码的基因,经确证后将该基因克隆到谷胱甘肽转移酶融合表达载体(pGEX-5x-1)中,构建了pGEX-5X-1-cdtA、cdtA1、cdtA2表达质粒,在大肠杆菌BL21中获得了相应表达。结果 pGEX-cdtA、cdtA1、cdtA2重组菌株具有明显的表达带,其分子量与预期的结果相同。结论成功构建了cdtA及其高抗原肽段的原核表达重组质粒,并表达出相应蛋白为其免疫原性分析及研制疫苗奠定了基础。Objective To express the cytolethal distending toxin cdtA and its peptids of highly antigenicity protein of Campylobacter jejuni in E.coli.Methods The encoding gene of cdtA,A1,A2 protein was amp lified from cultured Campylobacter jejuni via PCR and cloned into exp ression vector pGEX-5x-1 after identification theisequence.And they are expressed in BL21 of E.coli.Results Sequencing confirmed that encoding gene fragment of cdtA,A1,A2 protein were obtained and inserted into pGEX-5x-1correctly.The target protein expressed in E.coli was protected protein.Conclusion The recombinant plasmid was constructed and expressed in BL21 of E.coli successfully.It established groundwork for analysising immunogenicity and preparing Campylobacter jejuni DNA vaccine.

关 键 词：空肠弯曲菌 cdtA蛋白 重组质粒 

分 类 号：R378[医药卫生—病原生物学]