诸葛菜(Orychophragmus violaceus)查尔酮合成酶基因OvCHS的克隆与序列分析  

Isolation and sequence analysis of a chalcone synthase gene OvCHS from Orychophragmus violaceus

作　　者：倪雪莉[1] 黄余磊[1] 梁刚良[1] 鲍笑笑[1] 蒋明[1] 

机构地区：[1]台州学院生命科学学院,浙江临海317000

出　　处：《台州学院学报》2010年第3期42-48,共7页Journal of Taizhou University

基　　金：生命科学学院学生科研项目(09KY19);台州市科技计划项目(No.08XH02)资助

摘　　要：根据GenBank发布的基因序列,设计PCR引物,分别从诸葛菜(Orychophragmus violaceus)的花瓣基因组DNA和cDNA克隆到查尔酮合成酶基因,并定名为OvCHS,序列已上传至NCBI数据库,登陆号为EF408918。序列分析表明,OvCHS基因的基因组全长为1263 bp,具一个75 bp的内含子,编码区全长为1188 bp,编码395个氨基酸。与模式植物拟南芥(Arabidopsis thaliana)查尔酮合成酶基因AtCHS比较发现,两基因编码区有135个碱基不同,相似性为88.64%,氨基酸序列中仅16个氨基酸残基的差异,相似性达95.95%。PCR primers were designed according to known gene sequences registered in GenBank.A chalcone synthase gene,designated OvCHS,was isolated from petal genomic DNA and cDNA of Orychophragmus violaceus,respectively.Gene sequence was submitted to NCBI GenBank with accession number of EF408918.Sequence analysis showed that genomic DNA of OvCHS was 1263 bp with an intron of 75 bp and a complete coding se-quence of 1188 bp in length encoding 395 amino acids.Sequence comparison with model organism Arabidopsis thalian...

关 键 词：诸葛菜 查尔酮合成酶基因 克隆 表达分析 

分 类 号：Q943.2[生物学—植物学]