蛇床子和补骨脂含药血清对乳腺癌高转移细胞MDA-MB-231BO与骨髓基质细胞ST2的影响  

Effects of serum containing Fructus Cnidii and Psoralea corylifolia on highly metastatic human breast cancer cell line MDA-MB-231BO and bone marrow stromal cell line ST-2

作　　者：刘胜[1] 宋晓耘[1] 吴春宇[1] 韩向晖[1] 刘玲琳[1] 刘建文[2] 徐祎春[2] 

机构地区：[1]上海中医药大学龙华医院中医外科,上海200032 [2]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《中西医结合学报》2010年第9期878-882,共6页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.30772811);上海市教育委员会"曙光跟踪"资助项目(No.08GG12)

摘　　要：目的:探讨不同比例蛇床子和补骨脂配伍对乳腺癌高转移细胞株MDA-MB-231BO及骨髓基质细胞株ST2的影响。方法:36只雌性SD大鼠按蛇床子和补骨脂用药比例随机分为4:0组、3:1组、1:1组、1:3组、0:4组和空白组。灌胃4d制备中药血清。用各药物血清培养MDA-MB-231BO细胞及ST2细胞后,甲基噻唑基四唑法检测MDA-MB-231BO细胞和ST2细胞的抑制率,细胞迁徙实验检测MDA-MB-231BO细胞迁徙能力,磷酸对硝基苯酯法检测ST2细胞的碱性磷酸酶(alkaline phosphatase,ALP)活性,矿化结节形态计量法检测ST2细胞成骨能力。结果:与空白血清比较,各配伍比例蛇床子和补骨脂含药血清均能抑制MDA-MB-231BO细胞的迁移,其中1:1蛇床子和补骨脂配伍药物血清对MDA-MB-231BO细胞的抑制迁徙能力显著(P<0.01)。与空白组比较,蛇床子和补骨脂1:1配伍组ST2细胞ALP活性增强(P<0.05),ST2细胞矿化结节形成数量增加,差异有统计学意义(P<0.01)。结论:蛇床子和补骨脂含药血清能够抑制MDA-MB-231BO细胞增殖和转移,增强ST2细胞活性。Objective:To explore the effects of different proportions of Fructus Cnidii (Shechuangzi) and Psoralea corylifolia (Buguzhi) on highly metastatic human breast cancer cell line MDA-MB-231BO and bone marrow stromal cell line ST-2 in vitro.Methods:Thirty-six female SD rats were randomly divided into 6 groups to prepare the drug-medicated sera by administering with different proportions of Fructus Cnidii and Psoralea corylifolia,including 4︰0 group,3︰1 group,1︰1 group,1︰3 group,0︰4 group and control group. MDA-MB-231BO cells and ST-2 cells were cultured in Dulbecco’s modified Eagle’s medium containing drug-medicated serum. Inhibition rates of MDA-MB-231BO cells and ST-2 cells were measured by methyl thiazolyl tetrazolium (MTT) method; migration ability of MDA-MB-231BO cells was tested by a cell migration experiment; alkaline phosphatase activity (ALP) of ST-2 cells was measured by using 4-nitrophenyl phosphate disodium salt,and mineralized nodule formation of ST-2 cells was measured by alizarin red staining.Results:Sera contaning different proportions of Fructus Cnidii and Psoralea corylifolia inhibited the migration activity of MDA-MB-231BO cells as compared with the blank serum,and serum contaning Fructus Cnidii and Psoralea Corylifolia at proportion of 1︰1 had the best function (P<0.01). Fructus Cnidii and Psoralea corylifolia at ratio of 1︰1 also enhanced the ALP activity of ST2 cells (P<0.05) and increased the number of mineralized nodules of ST2 cells (P<0.01).Conclusion:Kidney-warming recipe of Fructus Cnidii and Psoralea corylifolia can inhibit proliferation and migration of MDA-MB-231BO cells and increase the activity of ST-2 cells.

关 键 词：乳腺肿瘤 乳腺癌高转移细胞MDA-MB-231BO 骨髓基质细胞ST2 蛇床子 补骨脂 

分 类 号：R285.5[医药卫生—中药学]