普鲁卡因的神经阻滞作用与pH值的关系研究  

Study of the relationship between the nerve block effect and the pH of procaine

作　　者：王茹[1] 花倩[1] 彭余楠[1] 吴昀蔚[1] 王振恒[1] 刘传飞[1] 徐浩[1] 曹俊平[2] 

机构地区：[1]徐州医学院麻醉学院,2006级2班江苏徐州221004 [2]徐州医学院麻醉药理学教研室,江苏徐州221004

出　　处：《徐州医学院学报》2010年第8期513-515,共3页Acta Academiae Medicinae Xuzhou

摘　　要：目的比较不同pH值的普鲁卡因溶液对蟾蜍坐骨神经动作电位的幅度和传导速度的影响,测定盐酸普鲁卡因溶液阻滞蟾蜍坐骨神经传导的半数有效pH值。方法制备30条离体蟾蜍坐骨神经干,分为6组,每组5根,分别用pH值为3、4、5、6、7、8的普鲁卡因溶液浸润,测定浸润前后动作电位的幅度和传导速度。分离26只蟾蜍左侧坐骨神经,用pH值为5、4、3.2、2.56、2.05的普鲁卡因溶液通过序贯法测定普鲁卡因溶液阻滞坐骨神经的半数有效pH值,观察指标为有无屈反射。结果 pH值为6的普鲁卡因溶液对蟾蜍坐骨神经动作电位的幅度和传导速度影响最大,普鲁卡因溶液阻滞坐骨神经的半数有效pH值的95%可信区间是2.28～2.77。结论不同pH值的普鲁卡因溶液对蟾蜍坐骨神经的阻滞作用不同。Objective To compare the effects of procaine under different pH conditions on the potential amplitude and propagation velocity of the compound action of toad sciatic nerve and to determine the 50% effective pH of procaine hydrochloride when the toad sciatic nerves were blocked.Methods 30 isolated toad sciatic nerve cords were divided into 6 groups and treated with procaine hydrochloride with pH of 3,4,5,6,7 and 8,respectively.The amplitude and propagation velocity of the compound action potential were recorded before and after procaine treatment.26 left sciatic nerves of toad were prepared and treated with procaine under pH conditions of 5,4,3.2,2.56,2.05 by sequential therapy to determine the 50% effective pH of procaine hydrochloride with the flexion reflex as a positive index.Results The amplitutd and the propagation velocity of the compound action potential of toad sciatic nerve changed significantly under procaine of pH 6.The confidence interval (95% ) of 50% effective pH procaine was 2.28-2.77.Conclusion The nerve block effects of procaine vary with different pH conditions.

关 键 词：坐骨神经 蟾蜍 动作电位 PH值 普鲁卡因 

分 类 号：R614[医药卫生—麻醉学]