抗大田软海绵酸单克隆抗体的制备与间接竞争ELISA的建立  

Preparation of monoclonal antibody against okadaic acid and establishment of ciELISA for detecting its residues

作　　者：王权[1,2] 闫叶娜[1] 黄克和[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]中国农业科学院上海兽医研究所兽医公共卫生实验室农业部动物寄生虫学重点实验室,上海200241

出　　处：《畜牧与兽医》2010年第8期1-5,共5页Animal Husbandry & Veterinary Medicine

基　　金：上海市技术标准专项(08DZ0505100)

摘　　要：用大田软海绵酸(okadaic acid,OA)与牛血清白蛋白(BSA)的偶联物OA-BSA作为免疫原,采用淋巴细胞杂交瘤技术制备OA单克隆抗体,并以纯化的抗体为探针建立了检测OA的快速、灵敏、简便的间接竞争ELISA(ciELISA)。回归方程和相关系数分别为:y=-0.3949x+0.6312,R2=0.9806;线性范围为0.3125~20 ng/mL;对OA的最低检出质量浓度为0.175 ng/mL;批内和批间变异系数分别为2.09%和3.27%;扇贝肉样的添加回收率为74.2%~80.8%;与鳍藻毒素(DTX1)的交叉反应(CR%)为51.82%,与石房蛤毒素(STX)无交叉反应。结果表明,建立了OA间接竞争ELISA检测方法,可用于海产品中OA残留检测。A hybridoma cell line secreting monoclonal antibody against okadaic acid(OA) was established after immunization of BALB/c mice with artificially synthesized OA-bovine serum albumin(OA-BSA) as antigen.A fast,sensitive and convenient indirect competitive enzyme linked immunosorbent assay(ciELISA) was set up.The standard curve for OA showed good linearity over the concentration range of 0.3125~20 ng/mL.The equation of linear regression was y=-0.3949x+0.6312.The coefficient correlation of the method was R2=0.98...

关 键 词：大田软海绵酸 单克隆抗体 间接竞争ELISA 

分 类 号：S917[农业科学—水产科学]