质子传递蛋白Bacteriorhodopsin颜色调谐的机理  

Mechanism of color tuning in proton transporter Bacteriorhodopsin

作　　者：陈德亮[1] 赵谦[1] 韩睿[1] 

机构地区：[1]中国科学院研究生院生命科学学院,北京100049

出　　处：《中国科学院研究生院学报》2010年第5期632-637,共6页Journal of the Graduate School of the Chinese Academy of Sciences

基　　金：中国科学院研究生院院长基金B(095102GN00)资助

摘　　要：直接与视黄醛短程作用的氨基酸残基的突变是Bacteriorhodopsin中颜色调谐的主要原因.我们发现:将第78位Ile替换为非天然残基R1,产生显著的颜色调谐.但R1在Gln75,Asn76,Thr128,Lys129和Leu61的替换,均未产颜色调谐.Bacteriorhodopsin光循环中间体的衰减表征发现:R1的替换使M412的衰减寿命显著延长.晶体结构发现:R1不与视黄醛发生短程作用,但可能直接与膜表面的"三体水分子"作用,并借助长程的氢键网络与视黄醛活性中心发生联系.The color tuning in Bacteriorhodopsin is mostly caused by mutated residues which directly interact with retinal.In the present study,we have found a significant color tuning at Ile78 substituted with a nonnative residue R1.However,no color tuning at Gln75,Asn76,Thr128,Lys129,or Leu61 was observed.In Bacteriorhodopsin photocycle,the lifetime of M412 was significantly prolonged after the substitution.The structural analysis suggested that R1 might have a direct contact with the "water trimer"and interact with...

关 键 词：颜色调谐 视黄醛 质子传递 光循环 

分 类 号：Q51[生物学—生物化学]