芪归解毒汤对硫酸镍诱导晶状体上皮细胞凋亡的抑制作用  

Inhibition effect on the apoptosis of NiSO_4 damaged lens epithelial cells by drug serum with Qigui Jiedu Decoction

作　　者：张兴儒[1] 张隆[2] 李青松[1] 俞彰[3] 周欢明[1] 刘龙民[4] 贾丹[1] 史才财[1] 

机构地区：[1]上海中医药大学附属普陀医院眼科,上海200062 [2]上海中医药大学附属普陀医院中心实验室 [3]上海复旦大学医学院电镜室 [4]上海中医药大学附属普陀医院中医科

出　　处：《中国中医眼科杂志》2010年第5期251-254,共4页China Journal of Chinese Ophthalmology

摘　　要：目的观察芪归解毒汤对硫酸镍诱导正常人晶状体上皮细胞(Lens epithelium cell,LEC)凋亡的影响。方法体外培养正常人晶状体上皮细胞,用血清药理学方法制备含芪归解毒汤药物血清,用药物血清和硫酸镍处理培养的LEC,采用流式细胞术检测LEC凋亡率;透射电子显微镜观察LEC超微结构改变和凋亡小体形成。结果硫酸镍组LEC凋亡率(26.99±2.58)高于中药组LEC(12.21±2.42),硫酸镍组和中药组LEC凋亡率均高于对照组(0.8±0.16),三组间差异有统计学意义(x2=-20.489,P<0.01),两两比较均有统计学意义(P<0.01)。透射电镜观察到硫酸镍组LEC各阶段均有细胞凋亡,可见到LEC凋亡后的继发性坏死。中药组LEC呈凋亡早期改变,只有少数LEC呈凋亡改变,未见有凋亡后继发性坏死的LEC。结论芪归解毒汤药物血清可有效地抑制硫酸镍诱导的LEC凋亡。OBJECTIVE To observe the Qigui Jiedu Decoction s effect on the apoptosis on damaged Lens Epithelial Cells induced by NiSO4. METHODS By methods of serum pharmacology,we fostered lens epithelia cells in vitro,divided them into NiSO4 and medicine group. The membrane of anterior lens capsule was taken with FCM method. The secondary necrosis and apoptosis body formation of LEC were observed with transmission electron microscope. RESULTS Qigui Jiedu Decoction could obviously inhibit LEC apoptosis. Its effect was ...

关 键 词：芪归解毒汤 晶体 上皮细胞 硫酸镍 血清药物学 

分 类 号：R285.5[医药卫生—中药学]