Simultaneous multi-parameter observation of Harring-tonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy  

作　　者：张春阳 李艳平 马辉 李素文 薛绍白 陈瓞延 

机构地区：Molecular and Nano Sciences Laboratory, Department of Physics, Tsinghua University, Beijing 100084, China[1] Department of Biology, Beijing Normal University, Beijing 100875, China[2]

出　　处：《Science China(Life Sciences)》2001年第4期383-391,共9页中国科学（生命科学英文版）

基　　金：the Tsinghua University Foundation for Basic Research and the Chinese Postdoctoral Foundation.

摘　　要：Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluores-cent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simulta-neously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ([Ca2+]i) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug- treating living cells at the level of single cell.Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simultaneously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ([Ca2+]i) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug- treating living cells at the level of single cell.

关 键 词：TWO-PHOTON LASER SCANNING microscopy  confocal LASER SCANNING microscopy  Harringtonine  HL-60 cells. 

分 类 号：Q503[生物学—生物化学]