Single nucleotide polymorphism analysis on chicken extracelluar fatty acid binding protein gene and its associations with fattiness trait  

作　　者：王启贵 李宁 邓学梅 连正兴 李辉 吴常信 

机构地区：National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China[1] National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China[2] College of Animal Science and Technology, China Agricultural University, Beijing 100094, China[3] College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China[4]

出　　处：《Science China(Life Sciences)》2001年第4期429-434,共6页中国科学（生命科学英文版）

基　　金：the National Basic Research Development Program and National Natural Science Foundation of China (Grant No. 39725022).

摘　　要：Fattiness is an important parameter to estimate meat quality, which has high heritability. In this experiment, F2 chickens derived from Broilers crossing to Silky were used to study the effect of extracellular fatty acid binding protein (EX-FABP) gene on abdominal fat accumulation. 1.6 kb of the 5′ region of the gene was amplified by six pairs of primers, and then single nucleotide poly-morphisms (SNPs) were detected by the technique of single strand conformation polymorphism (SSCP) and then confirmed by sequencing. There were four nucleotides variations found, A-G at -1807, C-A at -1805, T-C at -1011 and a C insertion at -1000 respectively. The result of least square analysis suggests that the birds with BB genotype defined by the second pair of primer have a higher abdominal fat weight and abdominal fat percentage than the birds with the other genotypes (AA and AB). It implied that EX-FABP gene could be a candidate locus or linked to a major gene to significantly affect abdominal fat traits in chicken.Fattiness is an important parameter to estimate meat quality, which has high heritability. In this experiment, F2 chickens derived from Broilers crossing to Silky were used to study the effect of extracellular fatty acid binding protein (EX-FABP) gene on abdominal fat accumulation. 1.6 kb of the 5' region of the gene was amplified by six pairs of primers, and then single nucleotide polymorphisms (SNPs) were detected by the technique of single strand conformation polymorphism (SSCP) and then confirmed by sequencing. There were four nucleotides variations found, A-G at -1807, C-A at -1805, T-C at -1011 and a C insertion at -1000 respectively. The result of least square analysis suggests that the birds with BB genotype defined by the second pair of primer have a higher abdominal fat weight and abdominal fat percentage than the birds with the other genotypes (AA and AB). It implied that EX-FABP gene could be a candidate locus or linked to a major gene to significantly affect abdominal fat traits in chicken.

关 键 词：F2 CHICKEN population  ABDOMINAL fat  EX-FABP  SNPs. 

分 类 号：Q525[生物学—生物化学]