Computational analysis and prediction for exons of PAC579 genomic sequence  

作　　者：黄弋 覃文新 万大方 赵新泰 顾健人 

出　　处：《Science China(Life Sciences)》2001年第5期533-540,共8页中国科学（生命科学英文版）

基　　金：The authors would like to thank Shanghai Genecore Company for finishing all the sequencing work.Thanks are due to Dr. Sun Fenyong, Dr. Ren Gongyi and Dr. Han Liwei for their excellent experimental work. Thanks also go to Prof. Gu Xiaocheng, Luo Jingchu

摘　　要：To isolate the novel genes related to human hepatocellular carcinoma (HCC), we sequenced P1-derived artificial chromosome PAC579 (D17S926 locus) mapped in the minimum LOH (loss of heterozygosity) deletion region of chromosome 17p13.3 in HCC, Four novel genes mapped in this genomic sequence area were isolated and cloned by wet-lab experiments, and the exons of these genes were located. 0-60 kb of this genomic sequence including the genes of interest was scanned with five different computational exon prediction programs as well as four splice site recognition programs. After analyzing and comparing the computationally predicted results with the wet-lab experiment results, some potential exons were predicted in the genomic sequence by using these programs.To isolate the novel genes related to human hepatocellular carcinoma (HCC), we se-quenced P1-derived artificial chromosome PAC579 (D17S926 locus) mapped in the minimum LOH (loss of heterozygosity) deletion region of chromosome 17p13.3 in HCC. Four novel genes mapped in this genomic sequence area were isolated and cloned by wet-lab experiments, and the exons of these genes were located. 0-60 kb of this genomic sequence including the genes of interest was scanned with five different computational exon prediction programs as well as four splice site recognition programs. After analyzing and comparing the computationally predicted results with the wet-lab experiment results, some potential exons were predicted in the genomic sequence by using these programs.

关 键 词：computational recognition and prediction EXON genomic sequence. 

分 类 号：Q523[生物学—生物化学]