Fast repair of purine deoxynucleotide radical cations by rutin and quercetin  

作　　者：赵晨阳 石益民 王文锋 贾忠建 姚思德 范波涛 郑荣梁 

机构地区：[1]School of Life Sciences, Lanzhou University, Lanzhou 730000, China [2]Laboratory of Radiation Chemistry, Shanghai Nuclear Research Institute, Chinese Academy of Sciences, Shanghai 201800, China [3]State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China [4]Institute de Topologie et de Dynamique des Systems, University Paris 7, Paris, 75005, France

出　　处：《Science China(Life Sciences)》2001年第6期610-617,共8页中国科学（生命科学英文版）

基　　金：This work was supported partly by the National Natural Science Foundation of China (Grant No.39870902), the Doctoral Programme of the Education Ministry of China, and the China-France Cooperative Research Sponsored by the Education Ministry of China.

摘　　要：Repair effects of rutin and quercetin on purine deoxynucleotide radical cations were studied using pulse radiolysis technique. On electron pulse irradiation of N2 saturated deoxynucleotide aqueous solution containing 20 mmol/L K2S2O8, 200 mmol/L f-BuOH and rutin or quercetin, the transient absorption spectra of the deoxynucleotide radical cations decayed quickly. At the same time, the spectra of flavonoid phenoxyl radicals formed within several dozen microseconds. The results indicated that deoxynucleotide radical cations can be repaired by flavonoids. The rate constants of the repair reactions were 3.8 × 108-4.4×108 mol-1 · L · s-1 and 1.3×108-1.8×108 mol-1 · L · s-1 for dAMP and dGMP radical cations, respectively.Repair effects of rutin and quercetin on purine deoxynucleotide radical cations were studied using pulse radiolysis technique. On electron pulse irradiation of N2 saturated deoxynucleotide aqueous solution containing 20 mmol/L K2S2O8, 200 mmol/L t-BuOH and rutin or quercetin, the transient absorption spectra of the deoxynucleotide radical cations decayed quickly. At the same time, the spectra of flavonoid phenoxyl radicals formed within several dozen microseconds. The results indicated that deoxynucleotide radical cations can be repaired by flavonoids. The rate constants of the repair reactions were 3.8×108-4.4×108 mol-1·L·s-1 and 1.3×108-1.8×108 mol-1·L·s-1 for dAMP and dGMP radical cations, respectively.

关 键 词：DNA damage deoxynucleotide radical cation REPAIR electron transfer RUTIN quercetin. 

分 类 号：Q526[生物学—生物化学]