Tissue engineering and peripheral nerve regeneration (Ⅲ)——Sciatic nerve regeneration with PDLLA nerve guide  

作　　者：王身国 侯建伟 贝建中 赵永强 

出　　处：《Science China Chemistry》2001年第4期419-426,共8页中国科学（化学英文版）

基　　金：Acknowledgements This work was supported by the High Technology Research and Development Program (Grant No. 863-715-002-0210) and the National Basic Science Research and Development Grants (Grant No. 973, G1999054306).

摘　　要：The biodegradation rate and biocompatibility of poly(d, / -lactide) (PDLLA) in vivo were evaluated. The aim of this study was to establish a nerve guide constructed by the PDLLA with 3-D microenvironment and to repair a 10 mm of sciatic nerve gap in rats. The process of the nerve regeneration was investigated by histological assessment, electrophysiological examination, and determination of wet weight recovery rate of the gastrocnemius muscle. After 3 weeks, the nerve guide had changed from a transparent to an opaque status. The conduit was degraded and absorbed partly and had lost their strength with breakage at the 9th week of postoperation. At the conclusion of 12 weeks, proximal and distal end of nerves were anastomosed by nerve regeneration and the conduit vanished completely. The results suggest that PDLLA conduits may serve for peripheral nerve regeneration and PDLLA is a sort of hopeful candidate for tissue engineering.The biodegradation rate and biocompatibility of poly(d, / -lactide) (PDLLA) in vivo were evaluated. The aim of this study was to establish a nerve guide constructed by the PDLLA with 3-D microenvironment and to repair a 10 mm of sciatic nerve gap in rats. The process of the nerve regeneration was investigated by histological assessment, electrophysiological examination, and determination of wet weight recovery rate of the gastrocnemius muscle. After 3 weeks, the nerve guide had changed from a transparent to an opaque status. The conduit was degraded and absorbed partly and had lost their strength with breakage at the 9th week of postoperation. At the conclusion of 12 weeks, proximal and distal end of nerves were anastomosed by nerve regeneration and the conduit vanished completely. The results suggest that PDLLA conduits may serve for peripheral nerve regeneration and PDLLA is a sort of hopeful candidate for tissue engineering.

关 键 词：poly(d. l-lactide) nerve conduit nerve guiding regeneration biodegradation BIOCOMPATIBILITY tissue engineering. 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]