Studies on the Basic Conformation of Lysozyme by Electrospray Ionization-Mass Spectrometry(ESI-MS)  

作　　者：GU Sheng SHEN Jin-can ZHUANG Zhi-xia WANG Xiao-ru 

机构地区：[1]Key Laboratory of Analytical Science of Ministry of Education,Department of Chemistry,Xiamen University,Xiamen 361005,P.R.China

出　　处：《Chemical Research in Chinese Universities》2001年第4期368-373,共6页高等学校化学研究(英文版)

基　　金：National Natural Science Foundation of China.

摘　　要：In the present work, 2-mercaptoethanol and dithiothreitol were used to hydrogenise the internal disulfide bond in lysozyme. The experimental results indicate that the charge distribution of the proteins are different in the reaction process. From the calculated molecular weight, the reduction process of the disulfide bond in the molecules can be described, and the number of the disulfide bonds in the molecule can also be determined.In the present work, 2-mercaptoethanol and dithiothreitol were used to hydrogenise the internal disulfide bond in lysozyme. The experimental results indicate that the charge distribution of the proteins are different in the reaction process. From the calculated molecular weight, the reduction process of the disulfide bond in the molecules can be described, and the number of the disulfide bonds in the molecule can also be determined.

关 键 词：Protein DISULFIDE Electrospray ionization Mass spectrometry 

分 类 号：O657.3[理学—分析化学]