青岛文昌鱼hedgehog及两个hedgehog-like片段的克隆  

CLONING OF HEDGEHOG GENE AND TWO HEDGEHOG-LIKE FRAGMENTS IN QINGDAO AMPHIOXUS

作　　者：张燕君[1] 梁恺龙[1] 黄向炜[1] 张红卫[1] 

机构地区：[1]山东大学生命科学学院,山东济南250100

出　　处：《山东大学学报（理学版）》2001年第2期228-234,共7页Journal of Shandong University(Natural Science)

基　　金：国家自然科学基金资助项目 ( 3 9670 3 57;3 0 0 70 0 94 ) ;高等学校博士学科点专项科研基金资助项目

摘　　要：采用RT PCR方法获得了青岛文昌鱼 2 4 97bp长的hedgehog基因片段 ,其所编码的氨基酸序列与多种生物hh基因家族成员的相应片段显示了较高的同源性 .同时获得 2个含有部分hedgehog基因片段的序列hedgehog like 1和hedgehog like 2 ,提示文昌鱼中发生hedgehog基因倍增过程和有多拷贝hh基因存在的可能性 .为研究hh基因的分子进化提供了线索 .The hedgehog family of intercellular signalling molecules have essential functions in the development of both Drosophila and vertebrate embryos.A 2497bp fragment of hedgehog gene of Qingdao amphioxus was isolated by Reverse Transcription polymerase chain reaction(RT PCR).The deduced amino acid sequence has a high level of identity to other hedgehog proteins'.Two hedgehog like fragments were also isolated at the meantime,each containing a partial hedgehog sequence.The results imply the possibilities that Qingdao amphioxus has undergone the duplication of hedgehog genes and that it contains not only one hedgehog family member.

关 键 词：文昌鱼 hedgehog基因 hedgehog-like 克隆 

分 类 号：Q754[生物学—分子生物学]