Key factors for causing poplar Ice Nucleation Active bacterial canker and its control techniques  

杨树冰核细菌溃疡病发病主导因素及防治技术(英文)

作　　者：项存悌[1] 董爱荣[1] 刘学峰 李淳 原树忠[3] 张景华 

机构地区：[1]东北林业大学 [2]黑龙江省森防站 [3]黑龙江省森林植物园 [4]黑龙江省阿城市林业局

出　　处：《Journal of Forestry Research》2001年第3期157-164,209,共9页林业研究（英文版）

基　　金：National Foundation of Ninth Five-Year Plan (No. 96-005-04-01-03).

摘　　要：The isolation, culture and the active determination of poplar ice nucleation active (INA) bacteria and the inoculation tests in laboratory and field were conducted, and the varieties, distribution and number of poplar INA bacteria and its pathoge-nicity and freezing injury property were determined. The study results showed that the INA bacteria widely spread on poplar in Northeast China and caused the frozen injury for poplar under the frost condition in Spring or Autumn, which was the key factor to induce INA bacterial canker. Through evaluation and investigation of different poplar varieties and inoculation tests, fine dis-ease-resistant varieties and strains of poplar suitable for Northeast China were selected. Further tests for strong seedling showed that burying cuttings in sand and covering with plastic film could effectively avoid the frostbite, frozen and drought damage, reduce INA bacteria infection, and promote poplar growth. INA bacterial canker was detected early by highly special-ized antiserums of INA bacteria and the agglutinated test of ring-shaped boundary surface. The inducers such as streptomycin, phenylmercuric acetae, salicylic acid and heat-killed bacteria to immerse cuttings, have obvious induced disease-resistant effect. Before poplar sprouted in early spring, through spraying the solution of frostbite agent, the control effect also was obvious.通过对杨树冰核细菌分离培养、活性测定和室内外接种试验,研究了冰核细菌的种类、分布、数量、致冻性和致病性,结果表明冰核活性(INA)细菌普遍存在于东北地区杨树上,在春秋季节霜冻条件下造成杨树冻害,是杨树冰核活性细菌溃疡病发生的主导因素。通过对不同杨树品种品评调查和人工接种试验,选出了适合东北不同地区的优良杨树抗病品种、品系。壮苗试验表明,将杨树插穗进行沙藏处理和地膜覆盖育苗可避免杨树霜冻害和旱害,减轻冰核细菌对杨树苗木的侵染,又能提高苗木的生长。利用强冰核活性细菌专化性抗血清技术,采用环状界面凝集法可早期检测杨树冰核细菌溃疡病。采用链霉素等诱导剂浸泡杨树插穗进行育苗,有明显的诱导抗病效果。在早春杨树萌芽前喷洒防冻保护剂对该病有一定防治效果。

关 键 词：CLC number  S763.1 S792.11 

分 类 号：S763.7[农业科学—森林保护学]