EFFECTS OF QUERCETIN ON CELL MORPHOLOGY AND EXPRESSION OF PML mRNA AND PROTEIN OF NB4 AND HL-60 CELLS  

作　　者：钟璐 陈芳源 韩洁英 邵念贤 欧阳仁荣 

出　　处：《Journal of Shanghai Second Medical University(Foreign Language Edition)》2001年第1期6-10,共5页上海第二医科大学学报（英文版）

基　　金：Partially supported by the National Natural Science Foundation ofchina (36970328)

摘　　要：Objective To investigate the effects of quercetin on cell morphology, expression of promye-locytic leukemia (PML) mRNA and PML protein localization of NB4, HL-60 cells. Methods Cells mor-phology assayed by Wright’ s stain, fluorescence stain, and PML mRNA expression by RT-PCR, PML protein localization by immuno-fluorescence. Results The typical apoptosis was found in NB4 and HL-60 cells after treatment with quercetin. Immuno-fluorescence analysis showed after treatment with quercetin, the fusion pro-tein disappeared in NB4 cells, PML protein relocated, then degraded, and that also seen in HL-60 cells. The expression of PML mRNA is not changed in quercetin-treated cells. Conclusion PML play the role of apop-tosis inducer in leukemia cells at the translational level, quercetin can inhibit the proliferation of leukemia cells, and induce NB4, HL-60 cells apoptosis.Objective To investigate the effects of quercetin on cell morphology, expression of promye-locytic leukemia (PML) mRNA and PML protein localization of NB4, HL-60 cells. Methods Cells mor-phology assayed by Wright' s stain, fluorescence stain, and PML mRNA expression by RT-PCR, PML protein localization by immuno-fluorescence. Results The typical apoptosis was found in NB4 and HL-60 cells after treatment with quercetin. Immuno-fluorescence analysis showed after treatment with quercetin, the fusion pro-tein disappeared in NB4 cells, PML protein relocated, then degraded, and that also seen in HL-60 cells. The expression of PML mRNA is not changed in quercetin-treated cells. Conclusion PML play the role of apop-tosis inducer in leukemia cells at the translational level, quercetin can inhibit the proliferation of leukemia cells, and induce NB4, HL-60 cells apoptosis.

关 键 词：quercetinN84 cellHL-60 cellpromyelocytic leukemia 

分 类 号：R3[医药卫生—基础医学]