Genetic typing of mitochondrial DNA in sheep (Ovis aries)  

作　　者：赵兴波 冯继东 李宁 王爱华 吴常信 

机构地区：[1]College of Animal Science and Technology, China Agricultural University, Beijing 100094, China [2]State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China

出　　处：《Progress in Natural Science:Materials International》2001年第12期937-940,共4页自然科学进展·国际材料（英文版）

基　　金：Project supported by the Major State Basic Research Development Program of China (No. G2000016100).

摘　　要：Using PCR-RFLP and DNA sequencing, this study confirmed that HinfI polymorphism in the ovine mitochondrial COI gene resulted from the T-C substitution at the nucleotide 234 but this mutation did not encode another amino acid, which was actually a synonymous mutation. This single nucleotide polymorphism can be used as gene typing marker for mitochondrial genome in the research of the interactions between mitochondria and nucleus, extranuclear gene effects, and as molecular discriminating marker for embryo or individual in the research area of gene transfer and animal cloning.Using PCR-RFLP and DNA sequencing, this study confirmed that HinfI polymorphism in the ovine mitochondrial COI gene resulted from the T-C substitution at the nucleotide 234 but this mutation did not encode another amino acid, which was actually a synonymous mutation. This single nucleotide polymorphism can be used as gene typing marker for mitochondrial genome in the research of the interactions between mitochondria and nucleus, extranuclear gene effects I and as molecular discriminating marker for embryo or individual in the research area of gene transfer and animal cloning.

关 键 词：SHEEP mtDNA typing single nucleotide polymorphism. 

分 类 号：Q52[生物学—生物化学]