混合血清ELISA筛检HIV—Ab的可行性研究  

THE FEASIBILITY STUDY ABOUT ELISASCREENING DETECTING HIV - Ab FROM POOLED SERUM

作　　者：邵景东[1] 曹祥芬[1] 王胜富[1] 

机构地区：[1]张家港出入境检验检疫局,江苏215633

出　　处：《旅行医学科学》2001年第3期35-37,共3页

摘　　要：目的际检测条件下,运用混合血清ELISA筛检HIV-Ab的效果。方法利用ELISA对6份不同浓度的HIV-Ab阳性样本在不同稀释倍数下测定OD值,找出最适混合比例;用最佳混合比例测定6份阳性标本作重复性试验;对336份待检血清样本进行逐一法和混合血清法检测HIV-Ab。结果混合样本为4份时,高、中、低浓度阳性标本的S/CO都呈阳性结果。该方法经重复性试验和临床实验,符合率为100%。结论开展艾滋病监测和流行病学调查时,混合血清ELISA筛检HIV-Ab是一种经济、有效的方法。PURPOSE According to understand the effect of detected HIV- Ab from pooled serum in real conditions. METHODS To detect OD value by ELISA in different dilution ofdifferent concentrations of 6 positive samples of HIV- Ab,then to take repeated test using best mixed proportion of 6 samples.To detect HIV- Ab from 336 serum samples by single serum and mixed serums. RESULT The S/CO of each 4 samples were positive results in high,medium and low concentrations.This coincidene rate of this method is 100% with repeated test and clinical examination. CONCLUSION ELISA screening detecting HIV - Ab from pooled serum is a economic and effective method for surveillance and survey of AIDS.

关 键 词：混合血清 HIV-AB 筛检 可行性研究 

分 类 号：R1[医药卫生—公共卫生与预防医学]