外源DNA直接导入玉米的分子育种技术研究初报  

A Preliminary Report on the Research of the Molecular Seeds Breeding Technology with DNA as the Out-Resource Led Directly into Corn

作　　者：冯世康[1] 陈成斌[2] 赖群珍 陈超君[1] 张平刚[1] 

机构地区：[1]广西大学,南宁530005 [2]广西农科院,南宁530007

出　　处：《广西农学报》2001年第4期16-18,共3页Journal of Guangxi Agriculture

摘　　要：用玉米作为受体 ,以水稻、甘蔗、象草、竹子等亲缘较远的植物DNA作为供体 ,用花粉管通道法、种子萌动浸渍法、受体花粉携带法、未成熟籽粒微注射法将供体DNA直接导入受体。发现玉米D1 代在质量性状和数量性状都产生变异 ,而其中以花粉管通道法效果最好 ,种子萌动浸渍法次之 ,注射法最差。Using corn as the receptor,the DNA of the comparatively far relationstip plants, i e rice, elephant grass, sugarcane and bamboo etc. as the donators, the DNA of the donators were directly derived into the receptor through the methods of pollen tube passage, seed budding immersion, receptor pollen carrying and micro-injection of immature seeds.The results showed that the variations in the D 1 generation of corn took place in the characteristics of quality and quantity. Of all the above mentioned methods the effect of the pollen tube passage method was the best one, the seed budding immersion method was in the second position while the injection method was the worst.

关 键 词：外源DNA 直接导入 分子育种 

分 类 号：S[农业科学]