胸腔积液CD14^+细胞表达白介素-16的研究  

EXPRESSION OF INTERLUKIN 16 IN THE CD14^+CELLS IN PLEURAL EFFUSION

作　　者：李剑军[1] 聂明[2] 韦巍[2] 兰会华[2] 莫武宁[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028 [2]广西医科大学

出　　处：《广西医科大学学报》2009年第6期852-855,共4页Journal of Guangxi Medical University

基　　金：广西科技厅课题(No.桂卫Z2006062);广西医科大学博士生启动基金资助项目(304201)

摘　　要：目的:探讨胸腔积液中CD14^+细胞能否合成和分泌白介素-16(IL-16)。方法:采用流式细胞术(Flow cytometry,FCM)检测细胞内细胞因子,即在胸腔积液中单个细胞水平上进行细胞膜表面抗原的染色和细胞内IL-16的测定;磁珠分选(Magneticcell sorting,MACS)法从胸腔积液中分离出CD14^+细胞,然后进行细胞培养并收集培养上清液,采用酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)测定IL-16的浓度。结果:胸腔积液中CD14^+细胞内IL-16显阳性的细胞百分率为(16.28±13.63)%(n=35);磁珠分选出胸腔积液CD14^+细胞,其培养上清液中IL-16的浓度为(58.51±25.38)ng/L(n=5)。结论:在一定条件下,CD14^+细胞(单核/巨噬细胞)有部分能合成和分泌IL-16。Objective:To investigate whether CD14^+ cells in the pleural effusion could synthesize and secrete IL-16.Methods:The intracellular IL-16 expression in the pleural effusion cells was detected by flow cytometry (FCM).The surface antigen on the cellular membrane and the intracellular IL-16 were stained by the correspondent antibodies.Meanwhile,the CD14^+ cells in the pleural effusion were separated by magnetic cell sorting(MCAS).Then,the cells separated were cultured in RPMI1640 with 10%FCS.At last,the superna...

关 键 词：白介素-16 流式细胞术 磁珠分选 CD14^+细胞 

分 类 号：R561[医药卫生—呼吸系统]