高效液相色谱法同时测定三氯异氰脲酸(粉)中三聚氰酸和三聚氰胺的含量  

DETERMINATION OF CYANURIC ACID AND MELAMINE IN TRICHLOROISOCYANURIC ACID (POWDER) BY HPLC

作　　者：于晓辉[1] 万仁玲[1] 欧阳林山[1] 杨秀玉[1] 王小慈[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《化学分析计量》2009年第6期31-33,共3页Chemical Analysis And Meterage

摘　　要：建立高效液相色谱法同时测定三氯异氰脲酸(粉)中三聚氰酸和三聚氰胺含量的方法。采用HILIC Silica色谱柱(150mm×4.6mm,5μm),以乙腈-10mmol/L乙酸铵水溶液(9∶1)为流动相,用紫外检测器在213nm波长处进行检测。三聚氰酸和三聚氰胺的浓度分别在9.22～92.2μg/mL和10.92～109.2μg/mL范围内与对应的峰面积呈良好的线性关系。三聚氰酸和三聚氰胺测定结果的相对标准偏差分别为0.3%、0.4%(n=6)。在80%、100%、120%3个添加水平下三聚氰酸的平均回收率分别为117.41%、119.85%、114.40%。A HPLC method for the determination of cyanuric acid and melamine in trichloroisocyanuric acid (powder) was developed. The HILIC Silica column (150 mm×4.6 mm, 5 μm) was used with acetonitrile-10 mmol/L ammonium acetate aqueous solution (9∶1) as mobile phase. The detection wavelength was 213 nm. The linearity range of cyanuric acid was 9.22-92.2 μg/mL (r=0.999 9,n=5), and the range of melamine was 10.92-109.2 μg/mL (r=0.999 6,n=5).The relative standard deviation of cyanuric acid and melamine detection result...

关 键 词：高效液相色谱 三聚氰酸 三聚氰胺 三氯异氰脲酸 

分 类 号：S948[农业科学—水产养殖]