川芎嗪对肝星状细胞增殖及分泌细胞外基质的影响  

Effect of Ligustrazine on Proliferation and Extracellular Matrix Secretion of Rat Hepatic Stellate Cells in Vitro

作　　者：刘燕[1] 刘凌云[2] 田伟[3] 

机构地区：[1]广州空军司令部医院内科 [2]湖南中医药大学第一附属医院药剂科 [3]95100部队卫生队

出　　处：《临床医学工程》2010年第6期34-35,共2页Clinical Medicine & Engineering

摘　　要：目的观察川芎嗪对大鼠肝星状细胞增殖及分泌细胞外基质分泌的影响。方法用不同浓度的川芎嗪处理大鼠肝星状细胞;以MTT比色法测定细胞的增殖;放射免疫法测定透明质酸和层粘连蛋白;酶联免疫吸附法(ELISA)测定I型胶原的水平。结果川芎嗪可剂量依赖性地抑制HSC增殖,不同程度抑制I型胶原、透明质酸和层粘连蛋白分泌。结论川芎嗪可显著抑制HSC的增殖及分泌细胞外基质。Objective To investigate the effects of ligustrazine on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro.Methods HSC-T6 were incubated with different concentration of ligustrazine for 24 hours.MTT colorimetry was used for assaying proliferation of HSC.Cytotoxicity was measured by LDH colorimetry.Type I collage was determined by ELISA.Hyaluronic acid and laminin were measured by radioimmunoassay.Results Addition of 1 ～ 20 mmol/L ligustrazine into culture medium had no c...

关 键 词：肝纤维化 肝星状细胞 细胞外基质 增殖 川芎嗪 

分 类 号：R285[医药卫生—中药学]