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机构地区:[1]同济大学附属同济医院分院,上海200092 [2]同济大学中医研究所
出 处:《浙江中医药大学学报》2010年第6期825-827,共3页Journal of Zhejiang Chinese Medical University
基 金:上海市科学技术发展基金项目(No:024119035)~~
摘 要:[目的]探讨颜氏活血复方对心肌细胞缺氧/复氧损伤的作用及机制。[方法]通过制备含药血清、培养乳鼠原代心肌细胞、复制心肌细胞缺氧/复氧模型,采用细胞免疫化学法及TUNEL法,检测活血复方对心肌细胞缺氧/复氧损伤后心肌细胞存活率、Fas蛋白表达以及细胞原位凋亡的影响。[结果]颜氏活血复方能显著增加心肌细胞缺氧/复氧后细胞存活率、降低Fas蛋白表达以及细胞原位凋亡指数,从而减轻心肌细胞损伤。[结论]颜氏活血复方对心肌细胞缺氧/复氧后心肌细胞损伤有一定的保护作用。[Objective] To investigate the effect of Yan-shi Huoxue Recipe on the cardiomyocytes during hypoxia-reoxygenation.[Methods] Through making the serum containing the Yan-shi Huoxue Recipe,culturing the primary neonatal mice cardiomyocytes,establishing the model of the injury during hypoxia-reoxygenation,we analyze the influence of Yan-shi Huoxue Recipe on the survival rate of cardiomyocardial cells,the expression of Fas protein and the apoptosis index.[Results] Yan-shi Huoxue Recipe can protect the injury during hypoxia-reoxygenation by enhancing the survival rate of cardiomyocardial cells,decreasing the Fas protein expression and the apoptosis index.[Conclusion] The results indicate that Yan-shi Huoxue Recipe has the obviously protective effect on the cardiomyocytes during hypoxia-reoxygenation.
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