牛体外受精早期胚胎发育基因差异表达的研究  

Gene expression of bovine preimplantation embryos derived from in vitro fertilization

作　　者：陈苏仁[1] 张鑫[1] 栗雪冰[1] 仓明[1] 刘东军[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,内蒙古呼和浩特010021

出　　处：《畜牧与兽医》2010年第9期12-19,共8页Animal Husbandry & Veterinary Medicine

基　　金：国家863项目(2008AA101007);国家基础科学人才培养基金(J0730648);内蒙古自然科学基金重大项目(200711020401)

摘　　要：应用mRNA差异显示技术,对牛卵母细胞、体外培养的牛早期8细胞期和囊胚期胚胎的基因表达进行了研究。选取4条mRNA表达量存在差异的基因条带进行测序和同源性分析,并利用RT-PCR技术粗略检测其在卵母细胞、8细胞和囊胚中的表达水平。结果表明:4个基因分别与核糖体蛋白S4,Y连接1(RPS4Y1)、末端脱氧核苷酰转移酶作用因子2(DNTTIP2)、剪接和多聚腺苷酸化特异因子3(CPSF3)和转录因子AP-2γ(TFAP2C)高度同源。RPS4Y1、DNTTIP2、CPSF3和TFAP2C在牛卵母细胞和早期胚胎发育过程中mRNA表达量存在时间性差异,可能与其参与不同的生理活动有关。The mRNA differential display of gene expression in bovine oocytes,8-cell and blastocyst stage embryos cultured in vitro was studied in the current project.Four differential expression fragments were selected for sequencing and alignment.The level of mRNA expression was grossly examined by RT-PCR method.The results indicated that four differential expression bands were highly homologous with ribosomal protein S4,Y-linked 1(RPS4Y1),deoxynucleotidyltransferase,terminal,interacting protein 2(DNTTIP2),cleavage ...

关 键 词：牛 MRNA差异显示 早期胚胎 

分 类 号：S823[农业科学—畜牧学]