农杆菌介导法将抗草甘膦基因EPSPS转入玉米自交系的研究  

Study on Agrobacterium tumefaciens Mediated Transformation of EPSPS Gene into Maize Inbred Line

作　　者：孙传波[1] 麻鹏达[1] 陶蕊[1] 郭嘉[1] 李海华[1] 孟凡梅[1] 曲文利[1] 李海华[1] 刘文国[2] 刘德璞[1] 袁英 

机构地区：[1]吉林省农业科学院生物技术研究中心,长春130033 [2]吉林省农业科学院玉米研究所,长春130033

出　　处：《玉米科学》2010年第6期24-26,30,共4页Journal of Maize Sciences

基　　金：转基因生物新品种培育科技重大专项(2009ZX08003-018B)

摘　　要：通过农杆菌介导法将抗草甘膦基因EPSPS转入玉米自交系H99中,同时研究了农杆菌浓度、侵染时间及共生培养条件等因素对转化频率的影响。结果表明:菌液浓度OD600为0.6、侵染时间20 min、共生培养时间3 d为最佳转化条件。获得的转基因植株经PCR分析鉴定,其中7株表现阳性,证明外源基因已经整合到玉米基因组中。The EPSPS gene was transferred into maize inbred line H99 by Agrobacterium tumefaciens mediated transformation.Factors influenced transformation efficiency was studied,including bacterial concentration,infection time and co-cultivation time.Results showed that bacterium concentration at OD600= 0.6,20 minutes infection time and co-culture 3 days were the optimal conditions.PCR analysis on transformation plants demonstrated that 7 of them were positive and the foreign gene was integrated into maize genome.

关 键 词：玉米 农杆菌介导 转化频率 EPSPS基因 

分 类 号：S51[农业科学—作物学]