Evaluation of cross-reactive antibody response to HVR1 in chronic hepatitis C  

作　　者：Bing-Shui Xiu  Xiao-Yan Feng Jing He  Guo-Hua Wang  He-Qiu Zhang  Xiao-Guo Song  Kun Chen  Shi-Gan Ling  Cui-Xia Zhu Xiang-Ying Zhang  Lai Wei  Hui-Ying Rao 

机构地区：[1]Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing 100850 China [2]National Institute for Control of Pharma-ceutical and Biological Products Beijing 100050 China [3]Hepatology Institute People Hospital Peking University,Beijing 100044,China

出　　处：《World Journal of Gastroenterology》2010年第35期4460-4466,共7页世界胃肠病学杂志（英文版）

基　　金：Supported by The National Natural Science Foundation of China, No 30500476;The National High-Tech Science Foundation of China, No 2008AA02Z434;National S and T Major Projects for Infectious Disease Control, No 2008ZX10002-013 and 2009ZX09103-621;Beijing Natural Science Foundation, No 7082048

摘　　要：AIM:To evaluate the presence and cross-reactive anti-bodies against hypervariable region 1(HVR1) in hepatitis C virus(HCV) infected patients and its relationship with the progression of the disease.METHODS:Sixteen representative HVR1 proteins selected from a unique set of 1600 natural sequences were used to semiquantitate the cross-reactivity of HVR1 antibodies in the sera of HCV patients.Fifty-five chronic HCV patients including 23 with asymptomatic mild hepatitis,18 with chronic hepatitis and 16 with liver cirrhosis patients were studied.RESULTS:The degree of the cross-reactivity of anti-HVR1 antibodies in 23 patients with mild asymptomatic hepatitis was 3.09 ± 2.68,which was signif icantly lower than in those with chronic hepatitis(5.44 ± 3.93,P < 0.05) and liver cirrhosis(7.44 ± 3.90,P < 0.01).No correlation was observed between the broadness of the cross-reactivity anti-HVR1 antibodies and patient's age,infection time,serum alanine aminotransferase activity,or serum HCV-RNA concentration.It was the breath of cross-reactivity rather than the presence of anti-HVR1 antibody in HCV sera that was associated with the progression of liver disease.CONCLUSION:The broadly cross-reactive HVR1 antibodies generated in natural HCV patients can not neutralize the virus,which results in persistent infection in patients with chronic hepatitis.AIM:To evaluate the presence and cross-reactive anti-bodies against hypervariable region 1(HVR1) in hepatitis C virus(HCV) infected patients and its relationship with the progression of the disease.METHODS:Sixteen representative HVR1 proteins selected from a unique set of 1600 natural sequences were used to semiquantitate the cross-reactivity of HVR1 antibodies in the sera of HCV patients.Fifty-five chronic HCV patients including 23 with asymptomatic mild hepatitis,18 with chronic hepatitis and 16 with liver cirrhosis patients were studied.RESULTS:The degree of the cross-reactivity of anti-HVR1 antibodies in 23 patients with mild asymptomatic hepatitis was 3.09 ± 2.68,which was signif icantly lower than in those with chronic hepatitis(5.44 ± 3.93,P < 0.05) and liver cirrhosis(7.44 ± 3.90,P < 0.01).No correlation was observed between the broadness of the cross-reactivity anti-HVR1 antibodies and patient’s age,infection time,serum alanine aminotransferase activity,or serum HCV-RNA concentration.It was the breath of cross-reactivity rather than the presence of anti-HVR1 antibody in HCV sera that was associated with the progression of liver disease.CONCLUSION:The broadly cross-reactive HVR1 antibodies generated in natural HCV patients can not neutralize the virus,which results in persistent infection in patients with chronic hepatitis.

关 键 词：Hepatitis C virus Hypervariable region 1 CROSS-REACTIVITY 

分 类 号：R512.63[医药卫生—内科学]