改进V形孔培养法对牛胚胎体外生产效果的影响  

Effects of the modified well of well culture system on the in vitro production of bovine embryos

作　　者：禹学礼[1,2] 栗颖华[2] 李国浩[2] 李晓霞[2] 昝林森[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]河南科技大学动物科技学院,河南洛阳471003

出　　处：《西北农林科技大学学报（自然科学版）》2010年第9期32-36,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十一五"科技支撑计划项目(2006BAD04A11);农业科技跨越计划项目(2007-23);洛阳市科技计划项目(030218;0903036A)

摘　　要：【目的】探索V形孔培养法(WOW)、改进V形孔培养法(mWOW)和常规微滴培养法对牛胚胎体外生产效果的影响。【方法】将牛卵母细胞和受精卵分别采用常规微滴培养法(对照组)、WOW和mWOW进行体外培养,对照组每个微滴中置入20～25枚卵母细胞或胚胎,WOW和mWOW组每个V形孔中置入1枚卵母细胞或胚胎,计算卵裂率、囊胚率及受精后第6,7,8天的囊胚总细胞数和囊胚孵化率。【结果】mWOW组的卵裂率、囊胚率及第6,7,8天囊胚的总细胞数、囊胚孵化率与常规微滴组差异不显著(P>0.05),但显著高于WOW组(P<0.05);3个组第6,7,8天获得的囊胚总细胞数均呈逐渐下降的趋势。【结论】以TCM-199为基础培养液,用mWOW可以完成牛胚胎体外生产的全过程,且比WOW培养胚胎具有更强的发育能力和更好的质量。体外培养时,发育越快的胚胎质量越高。【Objective】 The research was to study the effects of the well of well system(WOW),the modified well of well system(mWOW) and drop system on the development potential and quality of in vitro produced bovine embryos.【Method】 The WOW,mWOW and drop were prepared before experiments.Bovine oocytes and presumptive zygotes were cultured in drop(control),WOW and mWOW systems respectively.About 20-25 oocytes or embryos were cultured in every one drop for control group.Only one oocyte or embryo was cultured in every V...

关 键 词：V形孔培养方法 改进V形孔培养法 牛 胚胎 体外生产 

分 类 号：S82[农业科学—畜牧学]