枯草芽孢杆菌溶血相关基因yqxC的表达及功能鉴定  

Expression and functional characterization of a hemolysis-associated gene yqxCfrom Bacillus subtilis

作　　者：刘杰[1] 张琼[1] 房春红[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2010年第11期145-150,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学人才专项(Z111020825);西北农林科技大学博士后科研项目(K308020901)

摘　　要：【目的】确定枯草芽孢杆菌溶血相关基因yqxC的功能。【方法】采用PCR方法,扩增枯草芽孢杆菌溶血相关基因yqxC,构建GST融合表达载体,并在大肠杆菌中进行表达。使用含体积分数5%绵羊血的琼脂平板,检测可溶性融合蛋白GST-YqxC的溶血活性。此外,采用RT-PCR检测yqxC基因在枯草芽孢杆菌中的转录情况。【结果】获得的可溶性融合蛋白GST-YqxC,在绵羊血琼脂平板上显示出较强的溶血活力;通过RT-PCR,检测到yqxC基因可以在枯草芽孢杆菌中转录。【结论】枯草芽孢杆菌yqxC基因编码蛋白YqxC有较强的溶血活性,且能够发生转录,yqxC很可能是引起枯草芽孢杆菌溶血的基因之一。【Objective】The study was done to identify the hemolysis-associated gene yqxCfromBacillus subtilis.【Method】A hemolysis-associated gene yqxCwas amplified from the genomic DNA of B.subtilis by PCR.GST fusion expression vectors were constructed and fusion proteins were expressed in Escherichia coli.5% sheep blood agar plates were used to measure the hemolytic activity of the expressed soluble fusion protein.In addition,the transcription of yqxCwas studied by RT-PCR.【Result】The soluble fusion protein GST-YqxC sh...

关 键 词：枯草芽孢杆菌 溶血相关基因 yqxC 

分 类 号：Q93[生物学—微生物学]