巨型艾美耳球虫(Eimeria maxima)地理株研究Ⅰ.11株巨型艾美耳球虫的分离与基本特征  

Study on Geographical Strains of Eimeria maxima Ⅰ.Isolation and Biological Characterization of Eleven Geographical Strains of Eimeria maxima

作　　者：陶建平[1] 辛玲[2] 李文超[2] 戴亚斌[3] 沈永林[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]扬州大学畜牧兽医学院,江苏扬州225009 [3]中国农业科学院家禽科学研究所,江苏扬州225003

出　　处：《中国兽医学报》2004年第4期349-352,共4页Chinese Journal of Veterinary Science

基　　金：江苏省高校重点实验室开放课题 ( KJS0 10 5 6)

摘　　要：采用单卵囊分离技术 ,从江苏、安徽、山东、广东、福建和上海等省 (市 ) 10个地区的鸡场及美国的球虫病疫苗中获得了 11株纯种球虫。根据下列特征 :(1)卵囊的大小、形态和颜色 ;(2 )孢子囊的大小和形态 ;(3)形状指数 (卵囊长宽之比 ) ;(4 )虫体肠道中的寄生区 ;(5 )肉眼病变的性质 ;(6 )最早孢子发育时间 ;(7)最短的潜在期 ,对这 11株纯种球虫作了鉴定。结果表明 ,11株纯种球虫均为巨型艾美耳球虫 (Eimeria maxima Tyzzer,192 9)地理株。应用 SPSS软件系统 ,对各株进行描述性统计和方差分析 ,结果显示 ,11株间存在不同程度的差异 。Using signle oocyst infected techniques,eleven pure species were isolated from ten geographical regions in Jiangsu,Anhui,Shandong,Guangdong,Fujian and Shanghai in China and a coccidiosis vaccine made in USA,and all found to be Eimeria maxima(Tyzzer,1929) according to the following criterion:①size,shape and colour of oocyst;②size and shape of sporocyst;③shape index(length divided width);④location of coccidia in the small intestine;⑤location and appearance of lesions;⑥sporulation time;⑦prepatent period.Data including thickness of wall,length and width of oocyst and sporocyst,and shape index were subjected to analysis of variance.Results demonstrated that there are significant differences between the strains from USA and from China.

关 键 词：巨型艾美耳球虫 地理株 卵囊 分离 繁殖 

分 类 号：S852.723[农业科学—基础兽医学]