非竞争性毛细管电泳免疫分析乙肝表面抗原  

Non-competitive Immunoassay for Hepatitis B Surface Antigen Using Capillary Electrophoresis with Conductance Detection

作　　者：韦寿莲[1] 莫金垣[2] 蔡沛祥[2] 

机构地区：[1]肇庆学院轻化系,肇庆526061 [2]中山大学化学与化工学院,广州510275

出　　处：《分析化学》2004年第7期871-874,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金 (No .2 9675 0 3 3 );广西省科学基金 (No .0 2 3 60 3 7);广东省自然科学基金 (No .0 0 12 3 7);广西省自然科学基金(No .9912 0 0 3 )资助项目

摘　　要：用硫脲标记乙肝抗体 (抗 HBs) ,基于抗原 抗体高选择性识别 ,建立了非竞争性毛细管电泳免疫分析乙肝表面抗原的新方法。研究了混合温育时间、缓冲溶液酸度和浓度、进样时间的影响。在 5mmol/LTris 2 0mmol/LH3 BO3 3mmol/LEDTA(pH 7.0 )的运行缓冲溶液中 ,游离的标记抗体和抗原抗体复合物在15min内完全分离。HBsAg在 4 .0～ 5 0mg/L范围内与复合物的峰面积呈良好的线性关系 ;相对标准偏差小于 6 % ;检出限为 3.0mg/L。该方法用于乙肝病人血清和正常人血清中HBsAg的测定 ,结果令人满意。Using capillary electrophoresis linked with conductance detection, a novel highly selective assay using antigen-antibody recognition was developed for the determination of hepatitis B surface antigen ( HBsAg). Hepatitis B surface antibody(HBsAb) was chemically labeled with thiourea. The physico-chemical parameters of the immunoassay reaction and separation conditions of the CE were investigated. After 45 min in-cubatio, antigen-antibody complex was formed, which could be well separated with free labeled antibody in a 5 mmol/L Tris-20 mmol/L H3BO3-3 mmol/L EDTA buffer (pH 7.0) within 15 min,, There was excellent linearity between peak area of complex and concentration of HBsAg in the concentration range of 4.0 similar to 50 mg/L. Relative standard deviations was less than 6%. The detection limit was found to be 3.0 mg/L. This method has been applied to determine the HBsAg in human blood serum with satisfactory results.

关 键 词：乙肝表面抗原 非竞争性毛细管电泳 免疫分析 硫脲 乙型肝炎 

分 类 号：R446.6[医药卫生—诊断学]