微囊化hES/293细胞生物学性状研究  

Study on the biological characteristic of hES/293 cells encapsulated in ACA microcapsule.

作　　者：张鹏飞[1] 蔡善君[1] 詹文芳[1] 谢兵[1] 李红[1] 宿罡[1] 

机构地区：[1]遵义医学院附属医院眼科,遵义563003

出　　处：《贵州医药》2011年第3期203-206,共4页Guizhou Medical Journal

基　　金：贵州省科技厅自然基金资助项目[黔科合J字(2005)2061号]

摘　　要：目的检测海藻酸钠-壳聚糖-海藻酸钠(ACA)微囊化人内皮抑素/293(human En-dostatin/293,hES/293)细胞体外培养状况下的生物学性状。方法实验研究分为两组:A组:hES/293细胞未用ACA微囊包裹;B组:hES/293细胞用ACA微囊包裹,每组6个样本。采用聚电解质络合法制作ACA微囊化hES/293细胞,台盼兰染色计数微囊化细胞的存活率和细胞总数,MTT法测定细胞生长状况,流式细胞术检测细胞周期。结果在实验观察的14d内,hES/293细胞可在微囊内以细胞团的形式生长,囊内细胞总数随培养时间的延长而增加,但细胞存活率呈下降趋势;B组细胞在前3 d生长缓慢,第5 d以后生长速率基本上相当于A组;MTT法检测两组在1、3、5、7d的OD值差异无统计学意义(t1=0.208,P1=0.843;t3=0.447,P3=0.674;t5=0.917,P5=0.401;t7=1.956,P7=0.108);流式细胞术显示,B组细胞处于S期的百分比在第7天高于A组,差别有统计学意义(t=3.341,P=0.020),持续时间可达14d,且第14 d二者百分比差异有显著性(t=10.115,P=0.002)。结论微囊化hES/293细胞具有较高的增殖和代谢活性,且能较长时间保持此活性。Objective To study the growth and viability of human Endostatin/293(hES/293) cells in the Alginate-chitosan-Alginate(ACA) microcapsules.Methods Two groups were divided which were group A(hES/293 cells non-encapsulated in ACA microcapsule) and group B(hES/293 cells encapsulated in ACA microcapsule).Each group included 6 samples.The hES/293 cells encapsulated in ACA microcapsule were prepared by polyelectrolyte complexometry technology.The biological characters of hES/293 cells were observed by trypan blue st...

关 键 词：微囊 hES/293细胞 细胞活性 细胞周期 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]