柞蚕蛾粘液腺及其胶着物质的初步研究  

The Study of Adhesive Proteins in the Colleterial Glands of Antherea pernyi

作　　者：朱保健[1] 吴洁梅[1] 芦文奎[1] 刘朝良[1] 

机构地区：[1]安徽农业大学蚕业丝绸系,合肥230036

出　　处：《蚕业科学》2004年第2期176-179,共4页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目 (编号 3 0 3 710 88) ;安徽省自然科学基金项目 (编号 0 10 412 0 1)

摘　　要：测定了柞蚕品种克青和普通家蚕品种安农 2号卵表面胶着物质的含量 ,比较了二者的粘液腺的形状与大小 ,并采用SDS 聚丙烯酰胺凝胶电泳的方法鉴定了柞蚕蛾粘液腺分泌的胶着物质的分子量。结果表明 :普通家蚕品种卵表面胶着物质的含量约 0 0 37mg/粒 ,而柞蚕的约为 1 0 1mg/粒 ,是普遍家蚕品种卵的 30倍左右 ;柞蚕的粘液腺枝状部呈细长管状结构 ,分枝较少 ,而家蚕粘液腺的枝状部分枝较多 ,干状部不如柞蚕的干状部发达 ;柞蚕的粘液腺干状部长度平均约 1 8cm ,而家蚕的粘液腺干状部长度平均约 0 9cm ;柞蚕粘液腺中的分泌胶着物出现了 7条主要谱带 ,其分子量分别为 76、72、5 6、30、2 6、2 4、14kD。Colleterial glands is part of female reproductive system which lie in both sides of ovipositor and the adhesive proteins is stored in it.When laying eggs,the adhesive substance on the surface of eggs makes the eggs attach to the host plant.The content of adhesive proteins and the comparison about the colleterial glands between Antherea pernyi (Keqing) and Bombyx mori (Annong No.2) are carried on in the test. The molecular weight of the adhesive proteins in the colleterial glands of Antherea pernyi was determined by SDS-PAGE .The result shows that the molecular mass of the proteins in the colleterial glands of the proteins is about 76,56,30,26,24,14 kD.The content of adhesive proteins adhered to the eggs of Antherea pernyi,Bombyx mori are respectively 1.01 mg/egg and 0.037 mg/egg.Reservior region of the colleterial gland of Antherea pernyi is bigger than that of Bombyx mori.The length of reservior region is respectively 1.8 cm and 0.9 cm.

关 键 词：柞蚕 粘液腺 胶着物质 

分 类 号：S8851[农业科学—特种经济动物饲养]