雷帕霉素对球囊损伤大鼠颈总动脉VSMC迁移抑制及β_3整合素、MMP9表达的影响  

Effect of rapamycin on migration of vascular smooth muscle cell and the expression of β_3 interin,MMP-9 post balloon injury in rat carotid artery

作　　者：武衡[1] 汤显靖[1] 黄开亮[1] 成志[1] 

机构地区：[1]南华大学附属第一医院神经内科,湖南421001

出　　处：《脑与神经疾病杂志》2011年第1期1-5,共5页Journal of Brain and Nervous Diseases

基　　金：湖南省卫生厅医药卫生科研课题(B2004-092)

摘　　要：目的探讨雷帕霉素(Rapa)对大鼠颈总动脉球囊损伤后血管平滑肌细胞迁移的抑制作用及对β3整合素、基质金属蛋白酶9(MMP9)表达的影响。方法 40只大鼠随机分为球囊损伤组和雷帕霉素组。球囊导管损伤大鼠颈总动脉做成球囊损伤模型。雷帕霉素组术前3 d及术后28d连续肌注雷帕霉素0.5 mg·kg-1·d-1。28d后取目标血管段行HE染色行组织学观察和形态学分析,免疫组化分析雷帕霉素对VSMC的迁移及β3整合素、MMP9表达的影响。结果雷帕霉素组损伤段血管内膜厚度(I)、中膜厚度(M)及内膜增生程度(I/M)均较球囊损伤组小(P<0.05),同时雷帕霉素组新生内膜中β3整合素、MMP9阳性细胞数较球囊损伤组少(P<0.05)。结论雷帕霉素能抑制大鼠颈总动脉球囊损伤后VSMC迁移和内膜增生,其作用机制可能与其抑制MMP9、β3整合素的表达有关。Objective To investigate the effect of rapamycin on migration of vascular smooth muscle cell(VSMC) and the expression level of β3 interin,matrix metalloproteinase9(MMP9) in a rat model of restenosis after balloon injury carotid artery.Methods Fourty rats were randomly divided into two groups : balloon group : received balloon injury only,and rapamycin group: intramuscular rapamycin 3 days before injury and continued for 28 days at a dose a of 0.5 mg/kg.Animals were killed after 28 days.The injured artery se...

关 键 词：雷帕霉素 血管平滑肌细胞 Β3整合素 基质金属蛋白酶9 

分 类 号：R965[医药卫生—药理学]