小鼠成体骨骼肌卫星细胞提取及纯化方法的改进  

A novel method of isolation and purification for adult mice skeletal myoblasts

作　　者：朱铠[1] 过常发[1] 王春生[1] 赖颢 夏宇 

机构地区：[1]复旦大学附属中山医院心外科

出　　处：《中国分子心脏病学杂志》2011年第2期113-116,共4页Molecular Cardiology of China

基　　金：上海市浦江人才计划(10PJ1402000);教育部新教师基金(20090071120032)

摘　　要：目的建立一种快速高效的小鼠成体骨骼肌卫星细胞的提取、纯化的实验方法。方法通过布比卡因预处理激活肌卫星细胞,48小时后,以混合酶一步消化法及改良纯化法从成年骨骼肌中分离高纯度卫星细胞,并与普通差速贴壁法、反复差速贴壁法及Percoll非连续密度梯度离心法等纯化方法相比较。结果通过改良提取、纯化法所得到的骨骼肌卫星细胞纯度可达到(82.53±3.16)%,明显高于普通差速贴壁法、反复差速贴壁法及Percoll非连续密度梯度离心法(P<0.05),并具有很好的增殖及分化能力。结论通过采用改良成体骨骼肌卫星细胞提取及纯化法,能快速高效地获取高纯度肌卫星细胞。Objective: To establish a novel method of isolation and purification for adult mice skeletal myoblasts. Method: Bupivacaine was used for proliferation of mice skeletal myoblasts. Forty eight hours later, muscles were digested by mixed enzyme and skeletal myoblasts were purified by our novel method. After immunofluorescence cell staining, the novel method was compared with different adhesion time method, repeatedly different adhesion time method, incontinuous density Percoll gradient centrifugation technique...

关 键 词：骨骼肌卫星细胞 纯化 增殖 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]