轮状病毒LLR株VP7抗原表位的预测与验证  

Prediction and validation of rotavirus LLR strain VP7 antigen epitopes

作　　者：鱼轲[1] 魏至栋[1] 蒋琳[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2011年第1期10-14,共5页Progress In Microbiology and Immunology

摘　　要：在GenBank中检索A组轮状病毒不同血清型的VP7基因信息,在氨基酸水平上与G10血清型LLR株的VP7序列进行序列对比,分析其血清型特异的氨基酸保守序列位点。结合蛋白质二级结构预测理论方案,设计合成3条具有轮状病毒G10血清型特异性氨基酸序列的多肽。通过检测合成肽对轮状病毒免疫血清与LLR抗原的结合抑制,证实三条多肽均具备了LLR表位属性。Three synthesized peptides were produced by mimicking the antigenic epitopes of VP7 protein in rotavirus LLR strain by computer techniques,which were analyzed according to its bioinformatics,and on the basis of hydrophilicity,antigenicity,accessibility and flexibility.The results of block test showed that three synthesized peptides are of the properties of the antigen epitopes of VP7,LLR.

关 键 词：轮状病毒 抗原表位 

分 类 号：R392[医药卫生—免疫学]