固相化HEK细胞在无血清培养基中高效表达重组人活性蛋白C  

High expression of rhAPC in serum free-medium by immobilized HEK cells

作　　者：李征 曾献武 

机构地区：[1]成都生物制品研究所,成都610023

出　　处：《微生物学免疫学进展》2011年第1期28-31,共4页Progress In Microbiology and Immunology

摘　　要：研究固相化HEK工程细胞的培养和产生rhAPC的水平。先将HEK细胞由贴壁适应为无血清悬浮培养,再用海藻酸钙固定,并在无血清培养基中悬浮培养(最高约为27mg/L)。固相细胞动态培养的表达水平高于细胞静态悬浮培养的表达水平(最高约为50mg/L)。固相细胞动态培养可实现细胞的高密度培养,得到较高的表达水平。The cultivation of immobilized HEK cells was performed and the expression level of rhAPC was detected.The adhere cells were adapted for suspension cultivation in serum-free medium,then these cells were immobilized with calcium alginate and cultured in serum-free medium.The results showed that rhAPC expression level of the immobilized cells is higher than that of non-immobilized cells.The highest expression level is 50mg/L,the high density culture of immobilized cells can be achieved.

关 键 词：固相化 HEK细胞 无血清培养 人活性蛋白C 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]