湖州铁线莲多糖的分离纯化及体外抗肿瘤活性研究  

Study on the Isolation,Purification and Anti-tumor Activity in vitro of the Polysaccharides Extracted from Clematis Huchouensis Tamura

作　　者：赵伟春[1] 李莉[1] 沈贵芳[1] 郭伟[1] 

机构地区：[1]浙江中医药大学生物工程学院,杭州310053

出　　处：《浙江中医药大学学报》2011年第2期240-243,共4页Journal of Zhejiang Chinese Medical University

基　　金：浙江省中医药科技计划项目(No:2008YB001);浙江中医药大学科研项目(No:200706)~~

摘　　要：[目的]分离纯化湖州铁线莲粗多糖(CHTP)并测定其体外抗肿瘤活性。[方法]采用DEAE-cellulose 52离子交换层析和Sephadex G-200凝胶过滤层析分离纯化CHTP;采用MTT法测定CHTP及经纯化后多糖(CHTPII-1)对体外培养的人肝癌细胞HepG2、人胃癌细胞MGC8-03和人白血病细胞K562等三种肿瘤细胞的抑制作用。[结果]经分离纯化获得多糖组分CHT-PII-1;在CHTP浓度为250～1000mg.L-1或CHTPII-1浓度为125～500mg.L-1的检测范围内,CHTP和CHTPII-1对HepG2、MGC8-03和K562三种细胞的抑制率均随着多糖浓度的增大而增大。除了MGC8-03细胞62.5mg.L-1 CHTPII-1处理组和K562细胞125mg.L-1 CHTP处理组的MTT检测OD值与阴性对照组间无显著差异外,其余各组的MTT检测OD值均显著或极显著低于阴性对照组。[结论]CHTP和CHTPII-1对HepG2、MGC8-03和K562等三种肿瘤细胞均有较强的抑制作用,且其抑制率与多糖浓度呈良好的量效关系。[Objective] To isolate and purify the polysaccharide extracted from C.huchouensis Tamura and study their vitro anti-tumor activities.[Methods] Polysaccharide from C.huchouensis,named CHTP,was separated and purified by DEAE-Cellulose 52 ion-exchange chromatograph and Sephadex G-200 gel chromatograph.The anti-tumor activities of CHTP and its purified products were studied by MTT colorimetry.Three tumor cells such as HepG2,MGC8-03 and K562 were selected to detect their inhibitory effect.[Results] A polysaccharide fraction,named CHTPII-1,was harvested after purified.When the concentration of CHTP was 250～1000 mg·L-1 or the concentration of CHTPII-1 was 125～500 mg·L-1,the inhibition rate of CHTP or CHTPII-1 on HepG2,MGC8-03 and K562 cells increased with the increase of the polysaccharide concentration.There were significant differences of the OD value between the polysaccharide treatments and negative control except for MGC8-03 cells treated with 62.5 mg·L-1 CHTPII-1 or K562 cells treated with 125mg·L-1 CHTP tested by MTT.[Conclusion] Both CHTP and CHTPII-1 can inhibit the proliferation of the three tumor cells.Moreover,the inhibition rate is positively correlated to the amount of polysaccharides in test concentration.

关 键 词：湖州铁线莲 分离纯化 多糖 体外抗肿瘤 

分 类 号：R2[医药卫生—中医学]