RP-HPLC梯度洗脱法测定四逆汤中乌头类生物碱的含量  

Determination of Aconite Root Alkaloid in Si-Ni Decoction by RP-HPLC Gradient Elution Method

作　　者：叶姣云[1] 赵宁[1] 陈苹苹[1] 李范珠[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《浙江中医药大学学报》2011年第2期289-291,共3页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金资助项目(90409012);浙江省自然科学基金资助(杰出青年团队项目)--李范珠研究团队(R207722)~~

摘　　要：[目的]用RP-HPLC梯度洗脱法测定四逆汤中乌头类生物碱的含量。[方法]用Magic C18AQ色谱柱;乙腈-水(0.1%三乙胺)二元梯度洗脱;流速1.0mL.min-1;检测波长235nm。[结果]新乌头碱、乌头碱和次乌头碱的线性范围分别为6.65～665μg.mL-1,6.05～605μg.mL-1和5.80～580μg.mL-1。该方法回收率新乌头碱为98.77%(RSD=0.41%),乌头碱为101.3%(RSD=1.55%),次乌头碱为97.48%(RSD=1.34%)。[结论]RP-HPLC梯度洗脱法能将乌头类生物碱很好地分离检测,提高了时效,减少了误差,结果表明该方法准确可靠,重现性好,结果稳定。[Objective]A new RP-HPLC gradient elution was studied for the determination of aconitine-type alkaloids in Sini Decoction.[Methods]Magic C18 AQ column was used with a mobile phase of acetonitrile-water(0.1% triethylamine)gradient elution;the wavelength of detecter was set at 235nm.[Result]The linear range of mesaconitine,aconitine and hypaconitine was 6.65~665μg·mL-1,6.05~605μg·mL-1,5.80~580μg·mL-1respectively,the average recovery rate was 98.77%(RSD=0.41%),101.3%(RSD=1.55%)and 97.48%(RSD=1.34%)respectively.[Conclusion]The method was rapid,accurate,sensitive and raliable.The results showed that the method can be used to control quality of Sini Decoction.

关 键 词：RP-HPLC梯度洗脱法 四逆汤 乌头类生物碱 含量测定 

分 类 号：R2[医药卫生—中医学]