半胱氨酰白三烯受体激动剂和拮抗剂对大鼠胶质瘤C6细胞分化的作用  

Effects of agonist and antagonist of cysteinyl leukotriene receptors on differentiation of rat glioma C6 cells

作　　者：蔡蓓蕾[1] 王欣欣[1] 余舒莹[1] 黄雪琴[1] 张霞燕[1] 方三华[1] 卢韵碧[1] 张纬萍[1] 魏尔清[1] 

机构地区：[1]浙江大学医学院药理学系,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2011年第2期139-144,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助项目(30772561)

摘　　要：目的:探讨半胱氨酰白三烯受体在大鼠胶质瘤C6细胞的分化中的作用。方法:以激动剂LTD4、CysLT1受体拮抗剂孟鲁司特以及分化诱导剂forskolin处理C6细胞,观察细胞形态及GFAP蛋白表达变化。结果:Forskolin(10μmol/L)可诱导C6细胞形态变化及GFAP蛋白表达(细胞分化),而LTD4(0.1～100 nmol/L)不能诱导这些变化;单用孟鲁斯特(1μmol/L)无明显作用,但与LTD4(100 nmol/L)合用可诱导C6细胞分化。结论:CysLT2受体可能参与调节大鼠胶质瘤C6细胞的分化。Objective:To investigate the role of cysteinyl leukotriene(CysLT) receptors in the differentiation of rat glioma C6 cells. Methods:Rat glioma C6 cells were treated with the agonist LTD4,the CysLT1 receptor antagonist montelukast and the differentiation inducer forskolin.Cell morphology and GFAP protein expression were determined after treatments. Results:Forskolin(10 μmol/L) induced morphological changes and GFAP protein expression(cell differentiation) in C6 cells,but LTD4(0.1-100 nmol/L) did not induce these changes.Montelukast(1 μmol/L) alone did not affect C6 cell differentiation,while it induced the differentiation when combined with the LTD4(100 nmol/L). Conclusion:The CysLT2 receptor may modulate the differentiation of rat glioma C6 cells.

关 键 词：半胱氨酸 受体 白三烯 白三烯拮抗剂 乙酸盐类/药理学 大鼠胶质瘤C6细胞 细胞分化 半胱氨酰白三烯受体 激动剂 拮抗剂 

分 类 号：R739.4[医药卫生—肿瘤]