促红细胞生成素对体外培养间充质干细胞衰老的影响  

Effects of Erythropoietin on Aging of Cultured Mesenchymal Stem Cells in Vitro

作　　者：王妍妮[1,2] 陈龙 刘歧焕[2] 张有顺[3] 程范军 

机构地区：[1]武汉大学第一临床学院血液内科,湖北武汉430060 [2]湖北药学院附属东风医院血液内科,湖北十堰442008 [3]湖北药学院附属东风医院医学实验中心,湖北十堰442008 [4]华中科技大学协和医院血液病研究所,湖北武汉430022

出　　处：《郧阳医学院学报》2011年第2期116-118,123,共4页Journal of Yunyang Medical College

基　　金：湖北医药学院优秀中青年科技创新团队专项基金(2008CXX02);湖北省自然科学基金重点项目(2009CDA063);湖北省自然科学基金面上项目(2010CDB08006)

摘　　要：目的:研究促红细胞生成素(EPO)对体外培养的间充质干细胞(MSC)衰老的影响。方法:全骨髓细胞贴壁法培养大鼠间充质干细胞(MSC),EPO 1U/mL处理后,RT-PCR检测衰老相关基因OCT4、Nanog、SOX2 mRNA表达,30代后检测β-半乳糖苷酶活性,另设空白对照组。结果:随MSC增殖,对照组β-半乳糖苷酶活性较EPO组明显增高,群体倍增时间较EPO组长,EPO组OCT4、Nanog、SOX2 mRNA表达在一定时间内上调。结论:在长期培养过程中,促红细胞生成素能维持间充质干细胞未分化潜能,延缓衰老。O b je c tive To investigate the e ffects of e rythropoie tin(EPO) on aging ofmesenchyma l stem ce lls(MSCs) cu ltured in vitro. Me thodsMSCs was cultured by c lassical whole bone marrow adherence method. The expression of aging related genes OCT4, Nanog, SOX2mRNA in cells were de term ined w ith RT PCR a fter given EPO w ith a dose of 1 U /mL. The activ ity ofSA gal we re detected afte r 30 times of passage, meanwh ile, the con trol group were se t up. Re s ults The activity of SA ga l in control group was increased obvious ly than tha t ofEPO group with the proliferation ofMSCs. The popu la tion doubling time of control group was longe r than tha t of EPO group. The expression ofOCT4, Nanog, SOX2 mRNA in EPO group was up regu la ted dur ing ce rtain time. Conc lus ion E rythropoie tin couldma intain the und ifferentiating potential ofme senchyma stem cells in long term culture, delay the process of aging.

关 键 词：促红细胞生成素 间充质干细胞 衰老 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]