急性肾损伤小鼠肺组织损伤特点及水孔蛋白表达研究  

Characteristics of lung tissue injury and expression of aquaporins after acute kidney injury in mice

作　　者：王丽锋[1] 朱铭力[1] 吴蓓[1] 顾乐怡[1] 倪兆慧[1] 钱家麒[1] 戴慧莉[1] 严玉澄[1] 

机构地区：[1]上海交通大学医学院附属仁济医院肾脏科分子细胞(肾病)实验室,上海200127

出　　处：《上海交通大学学报（医学版）》2011年第4期411-416,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市卫生局科研课题(2009056)~~

摘　　要：目的研究急性肾损伤小鼠肺组织损伤特点及水孔蛋白(AQP)表达变化。方法 75只雄性C57BL/6小鼠随机分为假手术组(n=25)和急性肾损伤组(n=50),后者分别经缺血再灌注(I/R组,n=25)和双侧肾切除(BNx组,n=25)建立急性肾损伤模型。于造模后即刻(0 h)和2、4、6、24 h时点,分批处死动物留取肺脏组织。HE染色光学显微镜观察肺组织病理学改变,进行中性粒细胞浸润计数;计算肺湿质量与干质量比值(W/D);采用实时荧光定量聚合酶链反应(Real-Time PCR)技术和免疫组织化学方法检测肺组织AQP-1、AQP-5 mRNA和蛋白表达。结果 I/R组和BNx组小鼠分别从造模后2 h和4 h出现肺组织炎症细胞浸润、肺泡内出血和肺间质水肿;BNx组造模后4、6、24 h时点和I/R组造模后2、4、6、24 h时点小鼠肺脏组织中性粒细胞浸润计数均显著高于假手术组(P<0.05)。BNx组和I/R组小鼠造模后4、6、24 h时点的W/D均显著高于假手术组(P<0.05)。BNx组和I/R组小鼠造模后各时点肺组织AQP-1 mRNA和蛋白表达与假手术组比较差异均无统计学意义(P>0.05)。BNx组小鼠造模后2、4、6、24 h时点肺组织AQP-5 mRNA和蛋白表达均显著低于假手术组(P<0.05);I/R组小鼠造模后6 h和24 h时点肺组织AQP-5 mRNA和蛋白表达均显著低于假手术组(P<0.05)。结论肺水肿是急性肾损伤后急性肺损伤的典型表现,其发生可能与炎症反应和AQP-5表达的改变有关。Objective To investigate the characteristics of lung tissue injury and expression of aquaporins(AQP) after acute kidney injury in mice. Methods Seventy-five male C57BL/6 mice were randomly divided into sham operation group(n=25) and acute kidney injury group(n=50),and acute kidney injury models were established in acute kidney injury group by ischemia-reperfusion(I/R group,n=25) and bilateral nephrectomy(BNx group,n=25).Mice were sacrificed and lung tissues were collected 0 h,2 h,4 h,6 h and 24 h after model establishment.Pathological changes of lung tissues were observed by light microscopy with HE staining,leukocyte infiltration was counted,ratio of lung wet weight to dry weight(W/D) was calculated,and real-time quantitative polymerase chain reaction(Real-Time PCR) and immunohistochemical staining were employed to detect the expression of AQP-1 and AQP-5 mRNA and protein in lung tissues. ResultsInflammatory cell infiltration in lung tissues,alveolar hemorrhage and interstitial edema developed 2 h and 4 h after model establishment in I/R group and BNx group,respectively.The numbers of leukocytes in lung tissues 4 h,6 h and 24 h after model establishment in BNx group and 2 h,4 h,6 h and 24 h after model establishment in I/R group were significantly larger than those in sham operation group(P<0.05).W/D in BNx group and I/R group were significantly higher than those in sham operation group 4 h,6 h and 24 h after model establishment(P<0.05).The expression of AQP-1 mRNA and protein in lung tissues in BNx group and I/R group was not significantly different from that in sham operation group at each time point(P>0.05).The expression of AQP-5 mRNA and protein in lung tissues in BNx group was significantly lower than that in sham operation group at the time points of 2 h,4 h,6 h and 24 h(P<0.05),and the expression of AQP-5 mRNA and protein in lung tissues in I/R group was significantly lower than that in sham operation group at the time points of 6 h and 24 h(P<0.05). Conclusion Lung edema is the typical characteristics o

关 键 词：急性肾损伤 急性肺损伤 水孔蛋白 炎症 

分 类 号：R692[医药卫生—泌尿科学]