新型稳定中性电位水的理化性质及对院内感染常见多重耐药菌的灭活作用  

Physical and chemical properties of novel stabilized neutral potential water and its antimicrobial effect on common multi-drug resistant pathogens isolated from wards

作　　者：王文奎[1] 李作臻 张锋 倪语星[4] 韩立中[4] 

机构地区：[1]上海交通大学医学院附属瑞金医院灼伤整形科,上海200025 [2]青岛市城阳区人民医院检验科,青岛266100 [3]中国人民武装警察部队上海市总队医院放射科,上海201103 [4]上海交通大学医学院附属瑞金医院临床微生物科,上海200025

出　　处：《上海交通大学学报（医学版）》2011年第6期788-792,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市自然科学基金(09ZR1419200)~~

摘　　要：目的评估一种新型稳定中性电位水的理化性质,观察其对烧伤科院内感染常见多重耐药病原菌的灭活作用。方法选择新鲜配制(配制1个月)和保存8个月的新型稳定中性电位水作为待测样液,以有效氯含量、氧化还原电位(ORP)值和pH值等指标评估理化性质,观察长时间保存对其理化性质的影响;采用活菌培养计数的方法观察烧伤科院内感染常见的多重耐药病原菌(耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌和鲍曼不动杆菌流行克隆株)在添加新型稳定中性电位水的培养皿上的菌落生长情况,以无菌生理盐水代替中性电位水作为阳性对照进行平行试验。结果新鲜配制和保存8个月的新型稳定中性电位水待测样液的pH值为6.0~7.0,有效氯含量为50~100 mg/L,ORP值>800 mV,理化性质无明显变化。活菌培养计数结果显示:添加新鲜配制和保存8个月的新型稳定中性电位水的培养皿上均未见菌落生长。结论新型稳定中性电位水的刺激性小,理化性质稳定,灭菌效果理想,可作为烧伤科环境及与患者密切接触物品的消毒剂。Objective To evaluate the physical and chemical properties of a novel stabilized neutral potential water,and observe its antimicrobial effect on common multi-drug resistant pathogens isolated from burn wards.Methods Novel neutral potential water samples freshly prepared(prepared within 1 month) and preserved for 8 months were selected,parameters of effective chlorine content,oxidation-reduction potential(ORP) value and pH value were employed to evaluate the physical and chemical properties,and investigate the effects of long-term preservation on the physical and chemical properties.Colony growth of common multi-drug resistant pathogens(methicillin-resistant Staphylococcus aureus,Pseudomonas aeruginosa and Acinetobacter baumannii) isolated from burn wards in culture media with novel stabilized neutral potential water was observed by living bacterial counting,and sterilized normal saline was used to substitute neutral potential water as positive control for parallel test.Results The effective chlorine content,ORP value and pH value of novel stabilized neutral potential water samples freshly prepared and preserved for 8 months were 50 to 100 mg/L,>800 mV and 6.0 to 7.0 respectively,and there was no significant difference in physical and chemical properties between freshly prepared sample and that preserved for 8 months.Living bacterial counting revealed that no colony growth was observed in culture media with novel stabilized neutral potential water freshly prepared and preserved for 8 months.ConclusionNovel stabilized neutral potential water has less side effects on skin,stable physical and chemical properties and favorable antimicrobial effect,and can be an ideal disinfectant in burn wards.

关 键 词：稳定中性电位水 理化性质 多重耐药病原菌 活菌培养计数 

分 类 号：R446.5[医药卫生—诊断学]