妊娠期氰戊菊酯暴露对雄性仔鼠睾丸标志酶的影响  

Effects of fenvalerate on enzymes in testis of male offspring rats after maternal exposure in gestation

作　　者：李晓凤[1] 杨旸[1] 周义军[1] 

机构地区：[1]上海交通大学公共卫生学院,上海200025

出　　处：《上海交通大学学报（医学版）》2011年第8期1117-1120,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家大学生创新性实验计划(091024858);上海交通大学医学院基金(2008XJ006)~~

摘　　要：目的探讨妊娠期氰戊菊酯暴露对雄性仔鼠生殖系统的影响。方法将20只妊娠雌鼠随机分为对照组和不同剂量染毒组,其中对照组给予纯玉米油,染毒组分别给予2、10和50 mg/kg氰戊菊酯,于妊娠后第12~18天进行灌胃染毒。雄性仔鼠于出生后第35天处死,采用比色法测定睾丸标志酶酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)和γ-谷氨酰转肽酶(γ-GT)的活性,放射免疫法检测血清和睾丸匀浆中睾酮含量。结果 50 mg/kg染毒组雄性仔鼠的LDH活性明显低于对照组(P<0.05);2 mg/kg和10 mg/kg染毒组的γ-GT活性明显低于对照组(P<0.01);不同剂量染毒组的ACP和AKP活性与对照组比较差异均无统计学意义(P>0.05)。雄性仔鼠的血清和睾丸匀浆中睾酮含量随染毒剂量的增加而升高,但不同剂量染毒组与对照组比较差异均无统计学意义(P>0.05)。结论妊娠期氰戊菊酯暴露对雄性仔鼠的生殖系统有一定的影响,可明显影响睾丸标志酶的活性。Objective To investigate the effects of fenvalerate on reproductive system of male offspring rats after maternal exposure in gestation. Methods Twenty pregnant SD rats were randomly divided into three treated groups(treated with 2,10,and 50 mg/kg fenvalerate respectively on day 12 to 18 of gestation by means of intragastric injection) and control group(treated with corn oil).Male offspring rats were sacrificed on postnatal day 35,and the levels of serum testosterone(T) and testis homogenate T were determined by radioimmunoassay(RIA).Meanwhile,the activities of enzymes in testis such as acid phosphatases(ACP),alkaline phosphatase(AKP),lactate dehydrogenase(LDH) and gamma-glutamyl transpeptidase(γ-GT) were examined by colorimetry. Results Compared with control group,the activity of LDH was restrained in 50 mg/kg group(P<0.05),and those of γ-GT were increased in 2 mg/kg group and 10 mg/kg group(P<0.01).There was no significant difference in the activities of ACP and AKP between control group and treated groups(P>0.05).The levels of serum T and testis homogenate T were increased with different doses dependently,while there was no significant difference between control group and treated groups(P>0.05). Conclusion Exposure of fenvalerate in gestation has some influence on the reproductive system of male offspring rats,and can significantly affect the activities of enzymes in testis.

关 键 词：氰戊菊酯 妊娠 乳酸脱氢酶 - 谷氨酰转肽酶 睾酮 

分 类 号：R714.2[医药卫生—妇产科学]