结合珠蛋白基因多态性与冠状动脉狭窄程度的相关性研究  

The effect of Hp plasma concentration and polymorphism on the CHD patient

作　　者：王平[1] 刘玉清[1] 许莉[1] 刘红[1] 边文彦[1] 康健[1] 陈国良[1] 李一石[1] 

机构地区：[1]中国医学科学院北京协和医学院心血管病研究所阜外心血管病医院卫生部心血管药物临床研究重点实验室

出　　处：《中国分子心脏病学杂志》2011年第4期196-198,共3页Molecular Cardiology of China

基　　金："十一五"科技支撑计划"高血压遗传变异相关的药物安全性评价"课题(2009ZXO9501-32)

摘　　要：目的探讨结合珠蛋白基因多态性与冠状动脉狭窄严重程度的相关性。方法入选经冠状动脉造影确诊的冠心病患者103例,非冠心病患者83例作为对照组。采用免疫浊度分析仪定量测定血浆结合珠蛋白浓度,通过非变性聚丙烯酰胺凝胶电泳分型鉴定Hp基因型,分析Hp血浆浓度及Hp基因型与冠心病发生发展及冠状动脉狭窄严重程度的相关性。结果冠心病组血浆Hp浓度(93.98±53.10mg/L)明显高于对照组(68.88±43.83mg/dL),组间比较有显著性差异(P<0.05)。冠心病组与对照组间Hp基因型的分布频率无显著性差异(P>0.05)。但冠心病组间,Hp2-2基因型组的Gensini积分明显高于Hp1-1/Hp1-2基因型(p<0.05)。结论冠心病组血浆Hp浓度明显升高,Hp2-2基因型组的Gensini积分高于Hp1-1/Hp1-2基因型组,本研究结果提示HP血浆浓度与冠心病的发生发展有关,而Hp2-2基因型可能与冠心病患者冠状动脉狭窄的严重程度有关。Objective To study the relationship between the polymorphism of Haptoglobin (Hp) gene and the severity of coronary heart disease (CHD). Methods One hundred and three patients with CHD confirmed by coronary angiography were enrolled in this study and 83 subjects without CHD were selected as control. Concentration of Hp was measured by IMMAGE of Beckman Coulter,Inc (American Beckman company). Hp phenotypes of individuals were analyzed by the technique of the deformation polyacrylamide gel of electrophoresis. Results The Plasma levels of Haptoglobin in the CHD group (93.98±53.10 mg/dL) was higher than the control group (68.88±43.83mg/dL) (p<0.05). There were no significant differences in Hp Gene frequencies between two groups (P>0.05). In the CHD group, the Gensini integrate score of Hp2-2 genotypic individuals(68.14±43.34) is significant higher than the Hp1-1/1-2(51.79±32.35)( p<0.05). Conclusion: The plasma level of Hp is associated with the CHD. The Hp2-2 genotype has relationship with the stenosis degree of coronary artery in the extent of CHD of our small samples size study.

关 键 词：结合珠蛋白(Hp) Hp基因多态性 冠脉狭窄程度 

分 类 号：R541.4[医药卫生—心血管疾病]