大蒜根尖培养诱导不定芽研究  

Induce Adventitious Bud Garlic Root

作　　者：薛寒青[1,2] 王舰[1,2] 周淑兰[1,2] 高霞 

机构地区：[1]青海省农林科学院生物技术研究所,青海西宁810016 [2]青藏高原生物技术教育部重点实验室,青海西宁810016

出　　处：《北方园艺》2011年第9期120-122,共3页Northern Horticulture

基　　金：青海省科技厅资助项目(2006-N-515)

摘　　要：以青海省格尔木蒜脱毒后获得的脱毒小蒜为试材,研究不同培养基、蔗糖浓度、pH值和不同浓度激素配比对脱毒的格尔木大蒜根尖诱导不定芽的影响。结果表明:最佳培养基配比为MS培养基,pH 6.2,蔗糖20 g/L,BA 2 mg/L+2,4-D 0.1 mg/L。pH为6.2时诱导率达81.7%,蔗糖浓度20 g/L时诱导率达55%,激素配比为BA 2 mg/L+2,4-D 0.1 mg/L,诱导率达55%。Golmud small garlic affer virus-free was used as test materials in study on effect of different medium,sucrose concentration,pH,and different concentrations of hormones on induction adventitious bud of root tip from virus-free Golmud garlic.The results showed that the best medium ratio were MS medium,pH 6.2,sucrose 20 g/L,BA 2 mg/L+2,4-D 0.1 mg/L.Induction rate was 81.7% when pH was 6.2,induction rate was 55% when sucrose concentration was 20 g/L,induced rate was 55% when the hormone ratio were BA 2 mg/L+2,4-D 0.1 mg/L.

关 键 词：脱毒 大蒜 根尖 不定芽 

分 类 号：S6[农业科学—园艺学]