转拟南芥AtNHX5基因烟草的耐盐性研究  

Overexpression of AtNHX5 Improves Tolerance to Salt Stress in Tobacco

作　　者：林小洁[1] 李洪清[2] 

机构地区：[1]广东省阳江职业技术学院,广东阳江529566 [2]华南师范大学生命科学院广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《北方园艺》2011年第9期123-126,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(30170667)

摘　　要：采用农杆菌介导的叶盘法将来源于拟南芥的Na+/H+逆向转运蛋白基因AtNHX5导入烟草,经潮霉素筛选、PCR和RT-PCR检测,证明AtNHX5已导入烟草基因组并在转基因植株中表达。将转基因植株和非转基因(野生型)植株培养在含有300 mM NaCl的1/2MS培养基上,发现转基因植株的生长明显优于野生型。将在含有300 mM NaCl的1/2MS培养基中生长4周后的幼苗转移到不含NaCl的1/2MS培养基后,转基因植株快速恢复生长,而野生型植株则生长停滞。结果表明:超表达AtNHX5基因可以提高烟草的耐盐性。Na+/H+ antiporters have been shown to sequester excess Na+ into the vacuole and therefore represent attractive targets for application in molecular plant breeding of salt tolerance.In this study,transgenic tobacco plants overexpressing AtNHX5 showed higher salt tolerance than wild-type plants(WT) under high saline conditions.After salt stress recovery period,the growth of WT plants was obviously inhibited,while all the AtNHX5 transgenic plants recovered growth quickly.Taken together,the results indicated the feasibility of improving tolerance to salt stress in tobacco via genetic engineering,by introducing AtNHX5 into the genome of tobacco.

关 键 词：拟南芥AtNHX5 烟草 耐盐性 

分 类 号：S6[农业科学—园艺学]